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Alkaline phosphatase (intestinal) antibody (Biotin)

Anti-Alkaline phosphatase (intestinal) antibody (Biotin) used in Dot blot (Dot). GTX27323

Cat. No. GTX27323

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, Dot, ELISA

Reactivity

 Human
Package
1 mg ($399)
See all Alkaline phosphatase (intestinal) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
Dot Assay dependent
ELISA 1:4000-1:20000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Alkaline Phosphatase collected from human intestine

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Biotin

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alkaline phosphatase, intestinal , IAP

Cellular Localization

Cell membrane

Background

This polyclonal antibody reacts with alkaline phosphatase from human intestine showing a 60 kD band against this enzyme under reducing conditions of SDS-PAGE.

Database

Research Area

DATA IMAGES

Anti-Alkaline phosphatase (intestinal) antibody (Biotin) used in Dot blot (Dot). GTX27323

GTX27323 Dot Image

Dot blot analysis of human alkaline phosphatase using GTX27323 Alkaline phosphatase (intestinal) antibody (Biotin).
Lane 1 : 100 ng
Lane 2 : 33.3 ng
Lane 3 : 11.1
Lane 4 : 3.7 ng
Lane 5 : 1.23 ng
Dilution : 10 μg/mL

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399