APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Alkaline phosphatase (intestinal). The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2910038
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
alkaline phosphatase, intestinal , IAP
Background
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut. [provided by RefSeq, Dec 2014]
Database
Research Area
DATA IMAGES
![Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in IHC (Paraffin sections) (IHC-P). GTX636670](/templates/default/js/lightbox/images/loading.gif "Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in IHC (Paraffin sections) (IHC-P). GTX636670")
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GTX636670 IHC-P Image
Alkaline phosphatase (intestinal) antibody [HL1268] detects secreted Alkaline phosphatase (intestinal) protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Alkaline phosphatase (intestinal) stained by Alkaline phosphatase (intestinal) antibody [HL1268] (GTX636670) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636670 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (GTX636670) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636670 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (GTX636670) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636670 IHC-P Image
Alkaline phosphatase (intestinal) antibody [HL1268] detects secreted Alkaline phosphatase (intestinal) protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.Alkaline phosphatase (intestinal) stained by Alkaline phosphatase (intestinal) antibody [HL1268] (GTX636670) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636670 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (GTX636670) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636670 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (GTX636670) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for Alkaline phosphatase (intestinal) antibody [HL1268] (GTX636670). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Alkaline phosphatase (intestinal) antibody [HL1268] (GTX636670). Be the first to share your experience with this product.
![Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in IHC (Paraffin sections) (IHC-P). GTX636670](/upload/website/prouct_img/small/GTX636670/GTX636670_T-44529_20220114_IHC-P_M_22011718_286.jpg "Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in IHC (Paraffin sections) (IHC-P). GTX636670")
![Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in Western Blot (WB). GTX636670](/upload/website/prouct_img/small/GTX636670/GTX636670_T-44529_20220114_WB_M_tissue_22011718_636.jpg "Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in Western Blot (WB). GTX636670")
![Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in Western Blot (WB). GTX636670](/upload/website/prouct_img/small/GTX636670/GTX636670_T-44529_20220114_WB_22011718_694.jpg "Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in Western Blot (WB). GTX636670")
![Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in IHC (Paraffin sections) (IHC-P). GTX636670](/upload/website/prouct_img/small/GTX636670/GTX636670_T-44529_20220114_IHC-P_M_1_22011718_446.jpg "Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in IHC (Paraffin sections) (IHC-P). GTX636670")
![Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in Western Blot (WB). GTX636670](/upload/website/prouct_img/small/GTX636670/GTX636670_44599_20220715_WB_B_22071823_100.webp "Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in Western Blot (WB). GTX636670")
![Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in Western Blot (WB). GTX636670](/upload/website/prouct_img/small/GTX636670/GTX636670_T-44529_20220722_WB_M_tissue_22072519_971.webp "Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in Western Blot (WB). GTX636670")
![Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in IHC (Paraffin sections) (IHC-P). GTX636670](/upload/website/prouct_img/normal/GTX636670/GTX636670_T-44529_20220114_IHC-P_M_22011718_286.jpg "Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in IHC (Paraffin sections) (IHC-P). GTX636670")
![Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in Western Blot (WB). GTX636670](/upload/website/prouct_img/normal/GTX636670/GTX636670_T-44529_20220114_WB_M_tissue_22011718_636.jpg "Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in Western Blot (WB). GTX636670")
![Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in Western Blot (WB). GTX636670](/upload/website/prouct_img/normal/GTX636670/GTX636670_T-44529_20220114_WB_22011718_694.jpg "Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in Western Blot (WB). GTX636670")
![Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in IHC (Paraffin sections) (IHC-P). GTX636670](/upload/website/prouct_img/normal/GTX636670/GTX636670_T-44529_20220114_IHC-P_M_1_22011718_446.jpg "Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in IHC (Paraffin sections) (IHC-P). GTX636670")
![Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in Western Blot (WB). GTX636670](/upload/website/prouct_img/normal/GTX636670/GTX636670_44599_20220715_WB_B_22071823_100.webp "Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in Western Blot (WB). GTX636670")
![Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in Western Blot (WB). GTX636670](/upload/website/prouct_img/normal/GTX636670/GTX636670_T-44529_20220722_WB_M_tissue_22072519_971.webp "Anti-Alkaline phosphatase (intestinal) antibody [HL1268] used in Western Blot (WB). GTX636670")