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Alkaline phosphatase (intestinal) antibody

Orthogonal
Anti-Alkaline phosphatase (intestinal) antibody used in Western Blot (WB). GTX112100
Anti-Alkaline phosphatase (intestinal) antibody used in Western Blot (WB). GTX112100
Anti-Alkaline phosphatase (intestinal) antibody used in IHC (Paraffin sections) (IHC-P). GTX112100
Anti-Alkaline phosphatase (intestinal) antibody used in IHC (Paraffin sections) (IHC-P). GTX112100
Anti-Alkaline phosphatase (intestinal) antibody used in Western Blot (WB). GTX112100
Anti-Alkaline phosphatase (intestinal) antibody used in Western Blot (WB). GTX112100

Cat. No. GTX112100

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Alkaline phosphatase (intestinal) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alkaline phosphatase (intestinal). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949636

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alkaline phosphatase, intestinal , IAP

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor

Background

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Alkaline phosphatase (intestinal) antibody used in Western Blot (WB). GTX112100

GTX112100 WB Image

Orthogonal

Untreated (–) and treated (+) SW1116 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody (GTX112100) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Alkaline phosphatase (intestinal) antibody used in Western Blot (WB). GTX112100

GTX112100 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody (GTX112100) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Alkaline phosphatase (intestinal) antibody used in IHC (Paraffin sections) (IHC-P). GTX112100

GTX112100 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SG xenograft, using alkaline phosphatase(intestinal)(GTX112100) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Alkaline phosphatase (intestinal) antibody used in IHC (Paraffin sections) (IHC-P). GTX112100

GTX112100 IHC-P Image

Alkaline phosphatase (intestinal) antibody detects Alkaline phosphatase (intestinal) protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Alkaline phosphatase (intestinal) stained by Alkaline phosphatase (intestinal) antibody (GTX112100) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Alkaline phosphatase (intestinal) antibody used in Western Blot (WB). GTX112100

GTX112100 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody (GTX112100) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Alkaline phosphatase (intestinal) antibody used in Western Blot (WB). GTX112100

GTX112100 WB Image

Orthogonal

The data was published in the journal Mar Drugs in 2020. PMID: 32245246

REFERENCE

Application Reference

Cizkova K et al. Biomedicines 2021; 9 (9) When Activator and Inhibitor of PPARα Do the Same: Consequence for Differentiation of Human Intestinal Cells.
Application : ELISA
Reactivity : Human
Chandrarathna HPSU et al. Mar Drugs 2020; 18 (3) Marine Microalgae, Spirulina maxima-Derived Modified Pectin and Modified Pectin Nanoparticles Modulate the Gut Microbiota and Trigger Immune Responses in Mice.
Application : WB
Reactivity : Mouse
Mu Q et al. Microbiome 2017; 5 (1) : 73 Control of lupus nephritis by changes of gut microbiota.
Application : IHC-Fr
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169