GeneTex
United States (US)

Alkyl-DHAP synthase antibody

Overexpression
GTX101396_41577_WB_B_19011506_606.jpg
GTX101396_41577_20141017_WB_19011506_507.jpg

Cat No. GTX101396

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

MDA-MB-231 , human AGPS-transfected 293T

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Alkyl-DHAP synthase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Alkylglycerone Phosphate Synthase,Adap-S,Adas,Adhaps,Adps,Aldhpsy,Rcdp3,Agps

Cellular Localization

Peroxisome membrane

Background

This gene is a member of the FAD-binding oxidoreductase/transferase type 4 family. It encodes a protein that catalyzes the second step of ether lipid biosynthesis in which acyl-dihydroxyacetonephosphate (DHAP) is converted to alkyl-DHAP by the addition of a long chain alcohol and the removal of a long-chain acid anion. The protein is localized to the inner aspect of the peroxisomal membrane and requires FAD as a cofactor. Mutations in this gene have been associated with rhizomelic chondrodysplasia punctata, type 3 and Zellweger syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101396_41577_WB_B_19011506_606.jpg

GTX101396 WB Image

Overexpression

Alkyl-DHAP synthase antibody detects Alkyl-DHAP synthase protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human AGPS-transfected 293T cells
7.5 % SDS-PAGE
Alkyl-DHAP synthase antibody (GTX101396) dilution: 1:1000

GTX101396_41577_20141017_WB_19011506_507.jpg

GTX101396 WB Image

Alkyl-DHAP synthase antibody detects Alkyl-DHAP synthase protein by Western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkyl-DHAP synthase antibody (GTX101396) diluted by 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169