GeneTex
United States (US)

Alpha 1 Microglobulin antibody

GTX101068_40870_20160317_WB_19011506_200.jpg
GTX101068_40870_IHC_19011506_960.jpg
GTX101068_40870_IFA_19011506_374.jpg
GTX101068_40870_20160317_WB_H_plasma_19011506_652.jpg
GTX101068_40870_WB_19011506_894.jpg

Cat No. GTX101068

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:100000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T , A431 , human plasma , HepG2 conditioned medium

Predict Reactivity

Cat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha-1-Microglobulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Alpha-1-Microglobulin/Bikunin Precursor,A1M,Edc1,Hcp,Hi30,Iatil,Iti,Itil,Itilc,Uti,Ambp

Cellular Localization

Secreted

Background

This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101068_40870_20160317_WB_19011506_200.jpg

GTX101068 WB Image

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alpha 1 Microglobulin antibody (GTX101068) diluted at 1:100000.

GTX101068_40870_IHC_19011506_960.jpg

GTX101068 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using alpha-1-Microglobulin(GTX101068) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101068_40870_IFA_19011506_374.jpg

GTX101068 ICC/IF Image

alpha-1-Microglobulin antibody detects alpha-1-Microglobulin protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha-1-Microglobulin protein stained by alpha-1-Microglobulin antibody (GTX101068) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX101068_40870_20160317_WB_H_plasma_19011506_652.jpg

GTX101068 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Alpha 1 Microglobulin antibody (GTX101068) diluted at 1:5000.

GTX101068_40870_WB_19011506_894.jpg

GTX101068 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
10% SDS PAGE
GTX101068 diluted at 1:5000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169