HepG2 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alpha 1 Microglobulin antibody (GTX101068) diluted at 1:100000.

Immunohistochemical analysis of paraffin-embedded human hepatoma, using alpha-1-Microglobulin(GTX101068) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

alpha-1-Microglobulin antibody detects alpha-1-Microglobulin protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha-1-Microglobulin protein stained by alpha-1-Microglobulin antibody (GTX101068) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
10% SDS PAGE
GTX101068 diluted at 1:5000

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Alpha 1 Microglobulin antibody (GTX101068) diluted at 1:5000.