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Alpha 1 Microglobulin antibody

Orthogonal
Anti-Alpha 1 Microglobulin antibody used in Western Blot (WB). GTX101068
Anti-Alpha 1 Microglobulin antibody used in Western Blot (WB). GTX101068
Anti-Alpha 1 Microglobulin antibody used in Western Blot (WB). GTX101068
Anti-Alpha 1 Microglobulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101068
Anti-Alpha 1 Microglobulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101068

Cat. No. GTX101068

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Alpha 1 Microglobulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:100000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T , A431 , HepG2 conditioned medium , human plasma

Predict Reactivity

Cat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Alpha 1 Microglobulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171220

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alpha-1-microglobulin/bikunin precursor , A1M , EDC1 , HCP , HI30 , IATIL , ITI , ITIL , ITILC , UTI

Cellular Localization

Secreted

Background

This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Alpha 1 Microglobulin antibody used in Western Blot (WB). GTX101068

GTX101068 WB Image

Orthogonal

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alpha 1 Microglobulin antibody (GTX101068) diluted at 1:100000.

Anti-Alpha 1 Microglobulin antibody used in Western Blot (WB). GTX101068

GTX101068 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Alpha 1 Microglobulin antibody (GTX101068) diluted at 1:5000.

Anti-Alpha 1 Microglobulin antibody used in Western Blot (WB). GTX101068

GTX101068 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
10% SDS PAGE
GTX101068 diluted at 1:5000

Anti-Alpha 1 Microglobulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101068

GTX101068 ICC/IF Image

alpha-1-Microglobulin antibody detects alpha-1-Microglobulin protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha-1-Microglobulin protein stained by alpha-1-Microglobulin antibody (GTX101068) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Alpha 1 Microglobulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101068

GTX101068 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using alpha-1-Microglobulin(GTX101068) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

VN Patel et al. Matrix Biol 2024; S0945-053X (24) : S0945-053X(24)00090-8 Loss of 3-O-sulfotransferase enzymes, Hs3st3a1 and Hs3st3b1, reduces kidney and glomerular size and disrupts glomerular architecture
Application : WB
Reactivity : Mouse
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169