APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| 3-5μg/ml |
| Assay dependent |
|
Note :
IHC-P
Human Liver shows spotty cytoplasm staining in hepatocytes.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88657-PEP).
Product Note
This antibody is expected to recognise both reported isoforms (NP_000925.2; NP_001159393.1).
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-KDFLQSLKGFPRGDK, from the internal region of the protein sequence according to NP_000925.2; NP_001159393.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
serpin family F member 2 , A2AP , AAP , ALPHA-2-PI , API , PLI
Cellular Localization
Secreted
Background
This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Database
Research Area
DATA IMAGES
|
GTX88657 WB Image
WB analysis of human duodenum lysate using GTX88657 Alpha 2 Antiplasmin antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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|
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GTX88657 IHC-P Image
IHC-P analysis of human liver using GTX88657 Alpha 2 Antiplasmin antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
|
REFERENCE
There are currently no references for Alpha 2 Antiplasmin antibody, Internal (GTX88657). Be the first to share your publications with this product.
REVIEW
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