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Alpha fetoprotein / AFP antibody [3G3/2D3]

Overexpression
Anti-Alpha fetoprotein / AFP antibody [3G3/2D3] used in Western Blot (WB). GTX84948
Anti-Alpha fetoprotein / AFP antibody [3G3/2D3] used in Flow cytometry (FACS). GTX84948
Anti-Alpha fetoprotein / AFP antibody [3G3/2D3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84948
Anti-Alpha fetoprotein / AFP antibody [3G3/2D3] used in Western Blot (WB). GTX84948

Cat. No. GTX84948

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3G3/2D3

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($519)
See all Alpha fetoprotein / AFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alpha fetoprotein , AFPD , FETA , HPAFP

Cellular Localization

Secreted

Background

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Database

Research Area

DATA IMAGES

Anti-Alpha fetoprotein / AFP antibody [3G3/2D3] used in Western Blot (WB). GTX84948

GTX84948 WB Image

WB analysis of various cell lines using GTX84948 Alpha fetoprotein / AFP antibody [3G3/2D3].
Loading : 35 ug per lane

Anti-Alpha fetoprotein / AFP antibody [3G3/2D3] used in Flow cytometry (FACS). GTX84948

GTX84948 FACS Image

FACS analysis of HEK293T cells transfected with either Alpha fetoprotein / AFP plasmid(Red) or empty vector control plasmid(Blue) using GTX84948 Alpha fetoprotein / AFP antibody [3G3/2D3].

Anti-Alpha fetoprotein / AFP antibody [3G3/2D3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84948

GTX84948 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Alpha fetoprotein / AFP plasmid using GTX84948 Alpha fetoprotein / AFP antibody [3G3/2D3].

Anti-Alpha fetoprotein / AFP antibody [3G3/2D3] used in Western Blot (WB). GTX84948

GTX84948 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Alpha fetoprotein / AFP plasmid (Right) or empty vector (Left) for 48 hrs using GTX84948 Alpha fetoprotein / AFP antibody [3G3/2D3].
Loading : 5 ug per lane

REFERENCE

Application Reference

Chunhua Liu et al. Stem Cell Res Ther 2021; 12 (1) : 376 Hypoproliferative human neural progenitor cell xenografts survived extendedly in the brain of immunocompetent rats.
Liu J et al. Stem Cell Res 2020; 49 : 102010 Repair of acute liver damage with immune evasive hESC derived hepato-blasts.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519