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Alpha fetoprotein / AFP antibody [AFP-01]

Anti-Alpha fetoprotein / AFP antibody [AFP-01] used in Western Blot (WB). GTX23980

Cat. No. GTX23980

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AFP-01

Isotype

 IgG1

Application

 WB, IP, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all Alpha fetoprotein / AFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

purified alpha-Fetoprotein

Purification

Purified by precipitation and chromatography

Conjugation

Unconjugated

RRID

AB_367468

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AFP , AFPD , FETA , HPAFP , alpha fetoprotein

Cellular Localization

Secreted

Background

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Alpha fetoprotein / AFP antibody [AFP-01] used in Western Blot (WB). GTX23980

GTX23980 WB Image

WB analysis of HepG2 cell lysates using GTX23980 Alpha fetoprotein / AFP antibody [AFP-01].
Lane 1 : GTX23980 (reducing conditions)
Lane 2 : GTX23980 (non-reducing conditions)

REFERENCE

Application Reference

Given AM et al. J Pharm Biomed Anal 2012; 64-65 : 43327 Development and validation of an alpha fetoprotein immunoassay using Gyros technology.
Application : ELISA
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399