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Alpha fetoprotein / AFP antibody [C2]

Anti-Alpha fetoprotein / AFP antibody [C2] used in IHC (Paraffin sections) (IHC-P). GTX34407

Cat No. GTX34407

Host

Mouse

Clonality

Monoclonal

Clone Name

C2

Isotype

IgG1

Application

ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Specificity/Sensitivity

It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Alpha fetoprotein (AFP) purified from serum of a hepatoma patient

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Alpha Fetoprotein , Afpd , Feta , Hpafp , Afp

Background

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Alpha fetoprotein / AFP antibody [C2] used in IHC (Paraffin sections) (IHC-P). GTX34407

GTX34407 IHC-P Image

Formalin-fixed, paraffin-embedded human Fetal Liver stained with AFP Monoclonal Antibody (C2).

REFERENCE

There are currently no references for Alpha fetoprotein / AFP antibody [C2] (GTX34407). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 429