GeneTex
United States (US)

Alpha fetoprotein / AFP antibody [C3]

GTX34405_20170712_IHC-P_18121410_948.jpg

Cat No. GTX34405

Host Mouse
Clonality Monoclonal
Clone Name C3
Isotype IgG2a
Application IHC-P
Reactivity Human, Dog, Pig, Monkey
APPLICATION

Application Note

IHC-P:1-2ug/ml for 30 minutes at RT.

Calculated MW

69 kDa. ( Note )

Specificity/Sensitivity

It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Alpha fetoprotein (AFP) purified from serum of a hepatoma patient

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Alpha Fetoprotein,Afpd,Feta,Hpafp,Afp

Background

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX34405_20170712_IHC-P_18121410_948.jpg

GTX34405 IHC-P Image

Formalin-fixed, paraffin-embedded human Fetal Liver stained with AFP Monoclonal Antibody (C3).

Package List Price ($)
$ 329