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Alpha fetoprotein / AFP antibody [MBS-12]

Anti-Alpha fetoprotein / AFP antibody [MBS-12] used in IHC (Paraffin sections) (IHC-P). GTX34408

Cat No. GTX34408

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MBS-12

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($339)
See all Alpha fetoprotein / AFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 0.5-1μg/10⁶ cells in 0.1ml

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Specificity/Sensitivity

This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human Alpha feto protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AFP , AFPD , FETA , HPAFP , alpha fetoprotein

Cellular Localization

Secreted

Background

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Oct 2019]

Database

Research Area

DATA IMAGES

Anti-Alpha fetoprotein / AFP antibody [MBS-12] used in IHC (Paraffin sections) (IHC-P). GTX34408

GTX34408 IHC-P Image

IHC-P analysis of human fetal liver tissue using GTX34408 Alpha fetoprotein / AFP antibody [MBS-12].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339