APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 0.5-1μg/10⁶ cells in 0.1ml |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Specificity/Sensitivity
This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human Alpha feto protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
AFP , AFPD , FETA , HPAFP , alpha fetoprotein
Cellular Localization
Secreted
Background
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Oct 2019]
Database
Research Area
DATA IMAGES
![Anti-Alpha fetoprotein / AFP antibody [MBS-12] used in IHC (Paraffin sections) (IHC-P). GTX34408](/upload/website/prouct_img/middle/GTX34408/GTX34408_20200115_IHC-P_573_20011518_762.jpg "Anti-Alpha fetoprotein / AFP antibody [MBS-12] used in IHC (Paraffin sections) (IHC-P). GTX34408")
|
GTX34408 IHC-P Image
IHC-P analysis of human fetal liver tissue using GTX34408 Alpha fetoprotein / AFP antibody [MBS-12].
|
REFERENCE
There are currently no references for Alpha fetoprotein / AFP antibody [MBS-12] (GTX34408). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Alpha fetoprotein / AFP antibody [MBS-12] (GTX34408). Be the first to share your experience with this product.
![Anti-Alpha fetoprotein / AFP antibody [MBS-12] used in IHC (Paraffin sections) (IHC-P). GTX34408](/upload/website/prouct_img/small/GTX34408/GTX34408_20200115_IHC-P_573_20011518_762.jpg "Anti-Alpha fetoprotein / AFP antibody [MBS-12] used in IHC (Paraffin sections) (IHC-P). GTX34408")
![Anti-Alpha fetoprotein / AFP antibody [MBS-12] used in IHC (Paraffin sections) (IHC-P). GTX34408](/upload/website/prouct_img/normal/GTX34408/GTX34408_20200115_IHC-P_573_20011518_762.jpg "Anti-Alpha fetoprotein / AFP antibody [MBS-12] used in IHC (Paraffin sections) (IHC-P). GTX34408")
