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Alpha fetoprotein / AFP antibody [P5B8]

Anti-Alpha fetoprotein / AFP antibody [P5B8] used in Western Blot (WB). GTX15650
Anti-Alpha fetoprotein / AFP antibody [P5B8] used in Western Blot (WB). GTX15650
Anti-Alpha fetoprotein / AFP antibody [P5B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15650
Anti-Alpha fetoprotein / AFP antibody [P5B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15650

Cat. No. GTX15650

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 P5B8

Isotype

 IgG1

Application

 WB, ICC/IF, IP, ELISA, RIA

Reactivity

 Human
Package
100 μg ($399)
See all Alpha fetoprotein / AFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/ml
ICC/IF 5 μg/ml
IP Assay dependent
ELISA Assay dependent
RIA Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.01M Potassium Phosphate, 0.135M NaCl, 2.5mM KCl, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified AFP from Cord Blood

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alpha fetoprotein , AFPD , FETA , HPAFP

Cellular Localization

Secreted

Background

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Alpha fetoprotein / AFP antibody [P5B8] used in Western Blot (WB). GTX15650

GTX15650 WB Image

WB analysis of 20 ug of the indicated whole cell lysates using GTX15650 Alpha fetoprotein / AFP antibody [P5B8].
Dilution : 2 μg/ml

Anti-Alpha fetoprotein / AFP antibody [P5B8] used in Western Blot (WB). GTX15650

GTX15650 WB Image

WB analysis of 10 μg of whole cell extract from H9 ESCs, H9 ESC-derived hepatoblasts, ESC-derived hepatocyte-like cells, HepG2 cells, HepaRG cells, and primary human hepatocytes using GTX15650 Alpha fetoprotein / AFP antibody [P5B8].
Dilution : 1 μg/ml

Anti-Alpha fetoprotein / AFP antibody [P5B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15650

GTX15650 ICC/IF Image

ICC/IF analysis of permeabilized endoderm (derived from human ES cells) using GTX15650 Alpha fetoprotein / AFP antibody [P5B8].
Green : Primary antibody
Blue : cell nucleus
Dilution : 5 μg/ml

Anti-Alpha fetoprotein / AFP antibody [P5B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15650

GTX15650 ICC/IF Image

ICC/IF analysis of permeabilized endoderm (derived from human ES cells) using GTX15650 Alpha fetoprotein / AFP antibody [P5B8].
Green : Primary antibody
Blue : cell nucleus
Dilution : 5 μg/ml

REFERENCE

Application Reference

Y Zhang et al. Stem Cell Res 2023; : 103201 Human induced pluripotent stem cell line from fibroblasts (HEBHMUi015-A) and chondrogenic differentiation
Application : ICC/IF
Mingjing Zhang et al. Stem Cell Research 2023; Human Induced pluripotent stem cell (iPSC) line (HEBHMUi014-A) derived from a patient with Alzheimer's disease
Application : ICC/IF
Reactivity : Human
Kazutoshi Takahashi et al. PLoS Genet 2021; 17 (5) : e1009587 The pluripotent stem cell-specific transcript ESRG is dispensable for human pluripotency.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399