GeneTex
United States (US)

Alpha fetoprotein / AFP antibody [P5B8]

GTX15650_2069_WB_19011519_863.jpg
GTX15650_1503_WB_19011519_867.jpg
GTX15650_275_ICC-IF_19011519_286.jpg
GTX15650_274_ICC-IF_19011519_664.jpg

Cat No. GTX15650

Host Mouse
Clonality Monoclonal
Clone Name P5B8
Isotype IgG1
Application WB, ICC/IF, IP, ELISA, RIA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/ml
ICC/IF 5 μg/ml
IP Assay dependent
ELISA Assay dependent
RIA Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.01M potassium phosphate, 0.135M NaCl, 2.5mM KCl, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified AFP from Cord Blood

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Alpha Fetoprotein,Afpd,Feta,Hpafp,Afp

Cellular Localization

Secreted

Background

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX15650_2069_WB_19011519_863.jpg

GTX15650 WB Image

WB analysis of 20 ug of the indicated whole cell lysates using GTX15650 Alpha fetoprotein / AFP antibody [P5B8].
Dilution : 2 µg/ml

GTX15650_1503_WB_19011519_867.jpg

GTX15650 WB Image

WB analysis of 10 µg of whole cell extract from H9 ESCs, H9 ESC-derived hepatoblasts, ESC-derived hepatocyte-like cells, HepG2 cells, HepaRG cells, and primary human hepatocytes using GTX15650 Alpha fetoprotein / AFP antibody [P5B8].
Dilution : 1 µg/ml

GTX15650_275_ICC-IF_19011519_286.jpg

GTX15650 ICC/IF Image

ICC/IF analysis of permeabilized endoderm (derived from human ES cells) using GTX15650 Alpha fetoprotein / AFP antibody [P5B8].
Green : Primary antibody
Blue : cell nucleus
Dilution : 5 µg/ml

GTX15650_274_ICC-IF_19011519_664.jpg

GTX15650 ICC/IF Image

ICC/IF analysis of permeabilized endoderm (derived from human ES cells) using GTX15650 Alpha fetoprotein / AFP antibody [P5B8].
Green : Primary antibody
Blue : cell nucleus
Dilution : 5 µg/ml

Package List Price ($)
$ 319