APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin.
We do not recommend use of this product for Mouse,Rat,Bovine samples.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Alpha feto protein (AFP) Purified from serum of a hepatoma patient
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
AFP , AFPD , FETA , HPAFP , alpha fetoprotein
Cellular Localization
Secreted
Background
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Oct 2019]
Database
Research Area
DATA IMAGES
![Anti-Alpha fetoprotein / AFP antibody [SPM334] used in IHC (Paraffin sections) (IHC-P). GTX34406](/templates/default/js/lightbox/images/loading.gif "Anti-Alpha fetoprotein / AFP antibody [SPM334] used in IHC (Paraffin sections) (IHC-P). GTX34406")
|
GTX34406 IHC-P Image
IHC-P analysis of human fetal liver tissue using GTX34406 Alpha fetoprotein / AFP antibody [SPM334].
|
REFERENCE
There are currently no references for Alpha fetoprotein / AFP antibody [SPM334] (GTX34406). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Alpha fetoprotein / AFP antibody [SPM334] (GTX34406). Be the first to share your experience with this product.
![Anti-Alpha fetoprotein / AFP antibody [SPM334] used in IHC (Paraffin sections) (IHC-P). GTX34406](/upload/website/prouct_img/small/GTX34406/GTX34406_20200115_IHC-P_570_w_23060801_453.webp "Anti-Alpha fetoprotein / AFP antibody [SPM334] used in IHC (Paraffin sections) (IHC-P). GTX34406")
![Anti-Alpha fetoprotein / AFP antibody [SPM334] used in IHC (Paraffin sections) (IHC-P). GTX34406](/upload/website/prouct_img/normal/GTX34406/GTX34406_20200115_IHC-P_570_w_23060801_453.webp "Anti-Alpha fetoprotein / AFP antibody [SPM334] used in IHC (Paraffin sections) (IHC-P). GTX34406")