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Alpha fetoprotein / AFP antibody

Anti-Alpha fetoprotein / AFP antibody used in Western Blot (WB). GTX131311
Anti-Alpha fetoprotein / AFP antibody used in IHC (Paraffin sections) (IHC-P). GTX131311
Anti-Alpha fetoprotein / AFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131311

Cat. No. GTX131311

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Alpha fetoprotein / AFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Alpha fetoprotein / AFP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886460

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alpha fetoprotein , AFPD , FETA , HPAFP

Cellular Localization

Secreted

Background

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Alpha fetoprotein / AFP antibody used in Western Blot (WB). GTX131311

GTX131311 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alpha fetoprotein / AFP antibody (GTX131311) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Alpha fetoprotein / AFP antibody used in IHC (Paraffin sections) (IHC-P). GTX131311

GTX131311 IHC-P Image

Alpha fetoprotein / AFP antibody detects Alpha fetoprotein / AFP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
Alpha fetoprotein / AFP stained by Alpha fetoprotein / AFP antibody (GTX131311) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Alpha fetoprotein / AFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131311

GTX131311 ICC/IF Image

Alpha fetoprotein / AFP antibody detects Alpha fetoprotein / AFP protein by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Alpha fetoprotein / AFP stained by Alpha fetoprotein / AFP antibody (GTX131311) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Krasnova OA et al. Int J Mol Sci 2022; 23 (21) Prognostic Analysis of Human Pluripotent Stem Cells Based on Their Morphological Portrait and Expression of Pluripotent Markers.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169