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Alpha fetoprotein / AFP antibody

Anti-Alpha fetoprotein / AFP antibody used in Western Blot (WB). GTX31809
Anti-Alpha fetoprotein / AFP antibody used in IHC (Paraffin sections) (IHC-P). GTX31809
Anti-Alpha fetoprotein / AFP antibody used in IHC (Paraffin sections) (IHC-P). GTX31809
Anti-Alpha fetoprotein / AFP antibody used in Western Blot (WB). GTX31809
Anti-Alpha fetoprotein / AFP antibody used in IHC (Paraffin sections) (IHC-P). GTX31809
Anti-Alpha fetoprotein / AFP antibody used in IHC (Paraffin sections) (IHC-P). GTX31809

Cat. No. GTX31809

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Alpha fetoprotein / AFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

AFP antibody was raised against a 14 amino acid synthetic peptide near the center of human AFP. The immunogen is located within amino acids 340 - 390 of AFP.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alpha fetoprotein , AFPD , FETA , HPAFP

Cellular Localization

Secreted

Background

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Alpha fetoprotein / AFP antibody used in Western Blot (WB). GTX31809

GTX31809 WB Image

WB analysis of fetal human liver tissue lysate using GTX31809 Alpha fetoprotein / AFP antibody. The lane on the right was treated with GTX31809-PEP Alpha fetoprotein / AFP blocking peptide.
Loading : 10 μg
Dilution : 0.5 μg/mL

Anti-Alpha fetoprotein / AFP antibody used in IHC (Paraffin sections) (IHC-P). GTX31809

GTX31809 IHC-P Image

IHC-P analysis of rat liver tissue using GTX31809 Alpha fetoprotein / AFP antibody.
Working concentration : 20 μg/ml

Anti-Alpha fetoprotein / AFP antibody used in IHC (Paraffin sections) (IHC-P). GTX31809

GTX31809 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX31809 Alpha fetoprotein / AFP antibody.
Red : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml

Anti-Alpha fetoprotein / AFP antibody used in Western Blot (WB). GTX31809

GTX31809 WB Image

WB analysis of fetal human liver tissue lysate using GTX31809 Alpha fetoprotein / AFP antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-Alpha fetoprotein / AFP antibody used in IHC (Paraffin sections) (IHC-P). GTX31809

GTX31809 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX31809 Alpha fetoprotein / AFP antibody.
Working concentration : 2.5 μg/ml

Anti-Alpha fetoprotein / AFP antibody used in IHC (Paraffin sections) (IHC-P). GTX31809

GTX31809 IHC-P Image

IHC-P analysis of rat liver tissue using GTX31809 Alpha fetoprotein / AFP antibody.
Working concentration : 10 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399