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Aminoacylase 1 antibody [N2C3-2]

Anti-Aminoacylase 1 antibody [N2C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110348
Anti-Aminoacylase 1 antibody [N2C3-2] used in IHC (Paraffin sections) (IHC-P). GTX110348
Anti-Aminoacylase 1 antibody [N2C3-2] used in Western Blot (WB). GTX110348

Cat. No. GTX110348

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Aminoacylase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Aminoacylase 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949583

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aminoacylase 1 , ACY-1 , ACY1D , HEL-S-5

Cellular Localization

Cytoplasm

Background

Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Aminoacylase 1 antibody [N2C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110348

GTX110348 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Aminoacylase-1(GTX110348) antibody at 1:200 dilution.

Anti-Aminoacylase 1 antibody [N2C3-2] used in IHC (Paraffin sections) (IHC-P). GTX110348

GTX110348 IHC-P Image

Aminoacylase 1 antibody [N2C3-2] detects Aminoacylase-1 protein at cytoplasm on human placenta by immunohistochemical analysis.
Sample: Paraffin-embedded placenta.
Aminoacylase 1 antibody [N2C3-2] (GTX110348) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Aminoacylase 1 antibody [N2C3-2] used in Western Blot (WB). GTX110348

GTX110348 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX110348 diluted at 1:1000

REFERENCE

Application Reference

Sass JO et al. Metab Brain Dis 2015; Expanding the phenotype in aminoacylase 1 (ACY1) deficiency: characterization of the molecular defect in a 63-year-old woman with generalized dystonia.
Application : WB
Reactivity : Human
Sommer A et al. Biochim Biophys Acta 2011; 1812 (6) : 685-90 The molecular basis of aminoacylase 1 deficiency.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169