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Amphiphysin antibody, Internal

Anti-Amphiphysin antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89317
Anti-Amphiphysin antibody, Internal used in Western Blot (WB). GTX89317

Cat. No. GTX89317

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all Amphiphysin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
IHC-P 2-4μg/ml

Note :

IHC-P
Human Cerebellum shows nuclear and fibrous staning.
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89317-PEP).

Product Note

This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).

Predict Reactivity

Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KRKDESRISKAE, from the internal region of the protein sequence according to NP_001626.1; NP_647477.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

amphiphysin , AMPH1

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-Amphiphysin antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89317

GTX89317 IHC-P Image

IHC-P analysis of human cerebellum using GTX89317 Amphiphysin antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-Amphiphysin antibody, Internal used in Western Blot (WB). GTX89317

GTX89317 WB Image

WB analysis of mouse brain lysate using GTX89317 Amphiphysin antibody, Internal.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399