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Amphiphysin antibody

Anti-Amphiphysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32438
Anti-Amphiphysin antibody used in Western Blot (WB). GTX32438

Cat. No. GTX32438

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Amphiphysin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 340-620 of human AMPH (NP_001626.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

amphiphysin , AMPH1

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-Amphiphysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32438

GTX32438 ICC/IF Image

ICC/IF analysis of U-251 MG cells using GTX32438 Amphiphysin antibody.
Blue : DAPI
Dilution : 1:100

Anti-Amphiphysin antibody used in Western Blot (WB). GTX32438

GTX32438 WB Image

WB analysis of various sample lysates using GTX32438 Amphiphysin antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399