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Amphiregulin antibody [AT1D10]

Anti-Amphiregulin antibody [AT1D10] used in Western Blot (WB). GTX57585
Anti-Amphiregulin antibody [AT1D10] used in Flow cytometry (FACS). GTX57585

Cat. No. GTX57585

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT1D10

Isotype

 IgG1

Application

 WB, FACS

Reactivity

 Human
Package
100 μl ($399)
See all Amphiregulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:5000
FACS Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT1D10 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KLK5 protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

amphiregulin , AR , AREG , AREGB , CRDGF , SDGF , Amphiregulin

Cellular Localization

Membrane

Background

The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014]

Database

Research Area

DATA IMAGES

Anti-Amphiregulin antibody [AT1D10] used in Western Blot (WB). GTX57585

GTX57585 WB Image

WB analysis of PC-3 whole cell lysate using GTX57585 Amphiregulin antibody.
Loading : 40 μg
Dilution : 1:500~1:5000

Anti-Amphiregulin antibody [AT1D10] used in Flow cytometry (FACS). GTX57585

GTX57585 FACS Image

FACS analysis of LNCaP cells using GTX57585 Amphiregulin antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399