GeneTex

  • Country / Location Selection

United States (US)

Amphiregulin antibody

Anti-Amphiregulin antibody used in Western Blot (WB). GTX100986
Anti-Amphiregulin antibody used in IHC (Paraffin sections) (IHC-P). GTX100986
Anti-Amphiregulin antibody used in IHC (Paraffin sections) (IHC-P). GTX100986

Cat. No. GTX100986

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Amphiregulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

AREG-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Amphiregulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10616555

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

amphiregulin , AR , AREGB , CRDGF , SDGF

Cellular Localization

Membrane

Background

The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines. This encoded protein is associated with a psoriasis-like skin phenotype. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Amphiregulin antibody used in Western Blot (WB). GTX100986

GTX100986 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Amphiregulin antibody (GTX100986) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Amphiregulin antibody used in IHC (Paraffin sections) (IHC-P). GTX100986

GTX100986 IHC-P Image

Amphiregulin antibody detects Amphiregulin protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Amphiregulin antibody (GTX100986) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Amphiregulin antibody used in IHC (Paraffin sections) (IHC-P). GTX100986

GTX100986 IHC-P Image

The data was published in the journal Oncotarget in 2017. PMID: 28032593

REFERENCE

Application Reference

Yaojia Cheng YX et al. Int J Mol Med 2020; 45 (4) : 1163-1175 Aberrant expression of the UPF1 RNA surveillance gene disturbs keratinocyte homeostasis by stabilizing AREG.
Application : WB
Reactivity : Human
Khelwatty SA et al. Oncotarget 2019; 10 (18) : 1704-1715 Co-expression and prognostic significance of putative CSC markers CD44, CD133, wild-type EGFR and EGFRvIII in metastatic colorectal cancer.
Khelwatty S et al. Oncotarget 2017; 8 (5) : 7666-7677 The impact of co-expression of wild-type EGFR and its ligands determined by immunohistochemistry for response to treatment with * in patients with metastatic colorectal cancer.
Submit a Reference

REVIEW

There are currently no reviews for Amphiregulin antibody (GTX100986). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169