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Amylase antibody

Anti-Amylase antibody used in Western Blot (WB). GTX11728
Anti-Amylase antibody used in IHC (Paraffin sections) (IHC-P). GTX11728

Cat. No. GTX11728

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μg ($449)
See all Amylase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
IHC-P 0.5-1μg/ml
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1% Na₂HPO₄, 0.45% NaCl, 2.5% BSA

Preservative

0.025% Thimerosal, 0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Alpha Amylase 1(30-44aa HLFEWRWVDIALECE), different from the related rat and mouse sequences by one amino acid.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

amylase alpha 1C (salivary) , AMY1

Cellular Localization

Secreted

Background

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Amylase antibody used in Western Blot (WB). GTX11728

GTX11728 WB Image

WB analysis of various samples using GTX11728 Amylase antibody.
Lane 1 : human PANC-1 whole cell lysates
Lane 2 : rat pancreas tissue lysates
Lane 3 : mouse pancreas tissue lysates
Dilution : 0.5 μg/mL
Loading : 50μg of sample under reducing conditions

Anti-Amylase antibody used in IHC (Paraffin sections) (IHC-P). GTX11728

GTX11728 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX11728 Amylase antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Thimerosal.pdf
Package List Price ($)
$ 449