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Androgen Receptor (ARv7 Splice Variant) antibody [HL1239]

Recombinant antibody
Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636638
Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] used in Western Blot (WB). GTX636638
Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX636638
Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] used in Western Blot (WB). GTX636638

Cat. No. GTX636638

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1239

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P (cell pellet)

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Androgen Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Androgen Receptor (ARv7 Splice Variant). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910020

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

androgen receptor , AIS , AR8 , DHTR , HUMARA , HYSP1 , KD , NR3C4 , SBMA , SMAX1 , TFM

Cellular Localization

Nucleus,Cytoplasm

Background

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]

Database

Research Area

DATA IMAGES

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636638

GTX636638 ICC/IF Image

Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] detects Androgen Receptor (ARv7 Splice Variant) protein by immunofluorescent analysis.
Sample: 22RV1, LNCap and PC-3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Androgen Receptor (ARv7 Splice Variant) stained by Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] (GTX636638) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] used in Western Blot (WB). GTX636638

GTX636638 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted antibodies as follows:
Upper image : Androgen Receptor (ARv7 Splice Variant) antibody [HL1239], GTX636638 (1:1000)
Lower image : Androgen Receptor antibody [HL1049], GTX636021.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX636638

GTX636638 IHC-P (cell pellet) Image

Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] detects Androgen Receptor (ARv7 Splice Variant) protein at nucleus by immunohistochemical analysis.
Sample: PC-3 and 22RV1 FFPE Cell Pellet Block.
Green: Androgen Receptor (ARv7 Splice Variant) stained by Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] (GTX636638) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] used in Western Blot (WB). GTX636638

GTX636638 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] (GTX636638) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations