Androgen Receptor (ARv7 Splice Variant) antibody [HL1240]

Name: Androgen Receptor (ARv7 Splice Variant) antibody [HL1240]
Brand: GeneTex
SKU: GTX636639
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1240] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636639

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1240] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX636639

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1240] used in Western Blot (WB). GTX636639

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1240] used in IHC (Paraffin sections) (IHC-P). GTX636639

Cat. No. GTX636639

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1240
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-P (cell pellet)
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all Androgen Receptor products

Datasheet

View product citations for antibody GTX636639 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
IHC-P (cell pellet)	Assay dependent

Not tested in other applications.

Calculated MW

99 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Androgen Receptor (ARv7 Splice Variant). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910021

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

androgen receptor , AIS , AR8 , DHTR , HUMARA , HYSP1 , KD , NR3C4 , SBMA , SMAX1 , TFM

Cellular Localization

Nucleus,Cytoplasm

Background

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]

Database

Gene ID: 367 AR
UniProt: P10275 AR

Research Area

DATA IMAGES

GTX636639 ICC/IF Image

Androgen Receptor (ARv7 Splice Variant) antibody [HL1240] detects Androgen Receptor (ARv7 Splice Variant) protein by immunofluorescent analysis.
Sample: 22RV1, LNCap and PC-3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Androgen Receptor (ARv7 Splice Variant) stained by Androgen Receptor (ARv7 Splice Variant) antibody [HL1240] (GTX636639) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX636639 IHC-P (cell pellet) Image

Androgen Receptor (ARv7 Splice Variant) antibody [HL1240] detects Androgen Receptor (ARv7 Splice Variant) protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: PC-3 and 22RV1 cell FFPE Cell Pellet Block.
Green: Androgen Receptor (ARv7 Splice Variant) stained by Androgen Receptor (ARv7 Splice Variant) antibody [HL1240] (GTX636639) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636639 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted antibodies as follows:
Upper image : Androgen Receptor (ARv7 Splice Variant) antibody [HL1240], GTX636639 (1:1000)
Lower image : Androgen Receptor antibody [HL1049], GTX636021.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to dete+AD19ct the primary antibody.

GTX636639 IHC-P Image

Androgen Receptor (ARv7 Splice Variant) antibody [HL1240] detects Androgen Receptor (ARv7 Splice Variant) protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human prostate cancer.
Androgen Receptor (ARv7 Splice Variant) stained by Androgen Receptor (ARv7 Splice Variant) antibody [HL1240] (GTX636639) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636639 IHC-P Image

Androgen Receptor (ARv7 Splice Variant) antibody [HL1240] detects Androgen Receptor (ARv7 Splice Variant) protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human prostate cancer.
Androgen Receptor (ARv7 Splice Variant) stained by Androgen Receptor (ARv7 Splice Variant) antibody [HL1240] (GTX636639) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636639 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Androgen Receptor (ARv7 Splice Variant) antibody [HL1240] (GTX636639) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636639 WB Image

REFERENCE

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REVIEW