Immunohistochemistry (IHC): 1:100 – 1:200 dilution, Immunocytochemistry (ICC): 1:100 – 1:1000 dilution, Western Blot (WB): 1:500 – 1:1000 dilution.
This antibody reacts to androgen receptor splice variant 7 (AR-V7). No cross reactivity with wild type androgen receptor.
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
A peptide corresponding to AR splice variant 7
Protein A affinity purified from an animal origin-free culture supernatant
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
GTX33604 ICC/IF Image
Immunocytochemical staining of 22RV1 and PC-3 cells,using anti-AR-V7 rabbit monoclonal Antibody Clone RM7 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
GTX33604 IHC-P Image
Immunohistochemical staining of formalin fixed and paraffin embedded 22RV1, DU145 sections.
GTX33604 WB Image
Western Blot analysis of 22RV1, DU145, and PC-3 whole cell lysates, using anti-AR-V7 rabbit monoclonal Antibody Clone RM7, showed endogenous AR-V7 expressed in 22RV1 cells.