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Androgen Receptor (ARv7 Splice Variant) antibody [RM7]

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [RM7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33604
Anti-Androgen Receptor (ARv7 Splice Variant) antibody [RM7] used in IHC (Paraffin sections) (IHC-P). GTX33604
Anti-Androgen Receptor (ARv7 Splice Variant) antibody [RM7] used in Western Blot (WB). GTX33604

Cat No. GTX33604

Host Rabbit
Clonality Monoclonal
Clone Name RM7
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

Immunohistochemistry (IHC): 1:100 – 1:200 dilution, Immunocytochemistry (ICC): 1:100 – 1:1000 dilution, Western Blot (WB): 1:500 – 1:1000 dilution.

Calculated MW

99 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts to androgen receptor splice variant 7 (AR-V7). No cross reactivity with wild type androgen receptor.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

A peptide corresponding to AR splice variant 7

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Androgen Receptor,Ais,Ar8,Dhtr,Humara,Hysp1,Kd,Nr3C4,Sbma,Smax1,Tfm,Ar

Background

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Androgen Receptor (ARv7 Splice Variant) antibody [RM7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33604

GTX33604 ICC/IF Image

Immunocytochemical staining of 22RV1 and PC-3 cells,using anti-AR-V7 rabbit monoclonal Antibody Clone RM7 (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [RM7] used in IHC (Paraffin sections) (IHC-P). GTX33604

GTX33604 IHC-P Image

Immunohistochemical staining of formalin fixed and paraffin embedded 22RV1, DU145 sections.

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [RM7] used in Western Blot (WB). GTX33604

GTX33604 WB Image

Western Blot analysis of 22RV1, DU145, and PC-3 whole cell lysates, using anti-AR-V7 rabbit monoclonal Antibody Clone RM7, showed endogenous AR-V7 expressed in 22RV1 cells.

Package List Price ($)
$ 449