Recommended Starting Dilutions: For ELISA: Use at a concentration of 12.5 ng/ml. For WB: Use at a dilution of 1:2,000. Optimal working dilutions should be determined experimentally be the end user.
The Mouse anti-human AR antibody specifically detects targets derived from human recombinant protein at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
The immunogen corresponding to a region within amino acids 2 and 556 of human Androgen Receptor.
The IgG fraction of tissue culture supernatant was purified by Protein A/G affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
GTX16032 WB Image
WB analysis of (1) BSA, (2) recombinant protein AR and (3) protein marker using Androgen Receptor antibody [3C6-F12-F11] at a concentration of 0.5 μg/ml.