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Androgen Receptor antibody [AN1-15]

Anti-Androgen Receptor antibody [AN1-15] used in IHC (Paraffin sections) (IHC-P). GTX22742
Anti-Androgen Receptor antibody [AN1-15] used in IHC (Paraffin sections) (IHC-P). GTX22742
Anti-Androgen Receptor antibody [AN1-15] used in Western Blot (WB). GTX22742

Cat. No. GTX22742

Reference 4

Host

 Rat

Clonality

 Monoclonal

Clone Name

 AN1-15

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, Gel supershift assays, IHC

Reactivity

 Human, Mouse, Rat, Primate
Package
50 μg ($399)
See all Androgen Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:50-1:500
ICC/IF 5 μg/ml
IHC-P 2-4 μg/mL
IHC-Fr 2-4 μg/mL
IP Assay dependent
Gel supershift assays Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Product Note

This product does not cross-react with estrogen, progesterone, or glucocorticoid receptors.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A fusion protein corresponding to residues 331-572 of human androgen receptor.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_367517

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

androgen receptor , AIS , AR8 , DHTR , HUMARA , HYSP1 , KD , NR3C4 , SBMA , SMAX1 , TFM

Cellular Localization

Nucleus,Cytoplasm

Background

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]

Database

Research Area

DATA IMAGES

Anti-Androgen Receptor antibody [AN1-15] used in IHC (Paraffin sections) (IHC-P). GTX22742

GTX22742 IHC-P Image

IHC-P analysis of human prostate tissue using GTX22742 Androgen Receptor antibody [AN1-15].
A : isotype control
B : Primary antibody
C : Primary antibody with magnified section
Antigen retrieval : HEIR with a buffer (pH 6.2)
Dilution : 2 μg/ml

Anti-Androgen Receptor antibody [AN1-15] used in IHC (Paraffin sections) (IHC-P). GTX22742

GTX22742 IHC-P Image

IHC-P analysis of primate brain tissue using GTX22742 Androgen Receptor antibody [AN1-15].

Anti-Androgen Receptor antibody [AN1-15] used in Western Blot (WB). GTX22742

GTX22742 WB Image

WB analysis of 25 ug of T47D (lane 1), LNCaP (lane 2) and NIH-3T3 (lane 3) lysates using GTX22742 Androgen Receptor antibody [AN1-15].
Dilution : 1:100

REFERENCE

Application Reference

Prakasam R et al. Nat Commun 2023; 14 (1) : 603 LSD1/PRMT6-targeting gene therapy to attenuate androgen receptor toxic gain-of-function ameliorates spinobulbar muscular atrophy phenotypes in flies and mice.
Joel Shen et al. Differentiation 2018; (Epub) Immunohistochemical expression analysis of the human fetal lower urogenital tract.
Application : IHC
Reactivity : Human
Isaacson D et al. Differentiation 2017; (Epub) Renal Subcapsular Xenografing of Human Fetal External Genital Tissue – A New Model for Investigating Urethral Development.
Application : IHC-P
Reactivity : Human
Liu MC et al. PLoS One 2017; 12 (5) : e0178152 Inhibition of chronic prostate inflammation by hyaluronic acid through an immortalized human prostate stromal cell line model.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399