GeneTex
United States (US)

Androgen Receptor antibody [AR 441]

Androgen-receptor-antibody-AR-441-GTX29474-IHC-1_18121409_896.jpg

Cat No. GTX29474

Host Mouse
Clonality Monoclonal
Clone Name AR 441
Isotype IgG1
Application WB, IHC-P, IHC-Fr, IHC
Reactivity Human
APPLICATION

Application Note

Western blot: use at a dilution of 1 / 50 to 1 / 100. Immunohistochemistry: use at a dilution of 1 / 25 - 1 / 50 for 30 - 60 mins at RT(ABC method). Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 110 kDa, which corresponds to the predicted molecular weight of Androgen Receptor. For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval using 10mM citrate buffer pH6.0 before commencing with the IHC staining protocol.

Calculated MW

99 kDa. ( Note )

Positive Control

Prostrate Carcinoma

Specificity/Sensitivity

This antibody is specific to a protein of 110 kD, identified as androgen receptor. This antibody reacts with full length androgen receptor and also with the newly described A form of the receptor. This antibody does not cross react with estrogen, progesterone or glucocorticoid receptors.
PROPERTIES

Form

Liquid

Buffer

PBS, pH7.2 containing 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Antigen Species

Human

Immunogen

Synthetic peptide: STEDTAEYSPFKGGYTK, corresponding to amino acids 299-315 of Human Androgen Receptor.

Purification

purified immunoglobulin fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DHTR, SMAX1, P10275, TFM, Androgen receptor, HUMARA, AIS, NR3C4, AR, 367, KD, 176807, SBMA

Cellular Localization

Nuclear

Background

Androgens have a broad range of effects on the appearance and maintenance of male secondary sexual characteristics. Like other members of the steroid superfamily, the androgen receptor (AR) consists of an amino terminal modulating domain, a central DNA binding domain, a hinge region and a carboxy terminal ligand binding domain

Database

Research Area

DATA IMAGES
Androgen-receptor-antibody-AR-441-GTX29474-IHC-1_18121409_896.jpg

GTX29474 IHC Image

IHC image using GTX29474 - Detection of androgen receptor by IHC in human prostate

REFERENCE
Package List Price ($)
$ 319