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Androgen Receptor antibody [HL1049]

Recombinant antibody
Anti-Androgen Receptor antibody [HL1049] used in Western Blot (WB). GTX636021
Anti-Androgen Receptor antibody [HL1049] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636021
Anti-Androgen Receptor antibody [HL1049] used in IHC (Paraffin sections) (IHC-P). GTX636021

Cat No. GTX636021

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1049

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Androgen Receptor. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

androgen receptor , AIS , AR8 , DHTR , HUMARA , HYSP1 , KD , NR3C4 , SBMA , SMAX1 , TFM

Cellular Localization

Nucleus,Cytoplasm

Background

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]

Database

Research Area

DATA IMAGES

Anti-Androgen Receptor antibody [HL1049] used in Western Blot (WB). GTX636021

GTX636021 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Androgen Receptor antibody [HL1049] (GTX636021) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Androgen Receptor antibody [HL1049] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636021

GTX636021 ICC/IF Image

Androgen Receptor antibody [HL1049] detects Androgen Receptor protein at nucleus by immunofluorescent analysis.
Sample: 22RV1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Androgen Receptor stained by Androgen Receptor antibody [HL1049] (GTX636021) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-Androgen Receptor antibody [HL1049] used in IHC (Paraffin sections) (IHC-P). GTX636021

GTX636021 IHC-P Image

Androgen Receptor antibody [HL1049] detects Androgen Receptor protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human prostate cancer.
Androgen Receptor stained by Androgen Receptor antibody [HL1049] (GTX636021) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 369
$ 169