APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rabbit, Sheep, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Androgen Receptor. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2909933
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
androgen receptor , AIS , AR8 , DHTR , HUMARA , HYSP1 , KD , NR3C4 , SBMA , SMAX1 , TFM
Cellular Localization
Nucleus,Cytoplasm
Background
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]
Database
Research Area
DATA IMAGES
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GTX636021 IHC-P Image
Androgen Receptor antibody [HL1049] detects Androgen Receptor protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human prostate cancer.Androgen Receptor stained by Androgen Receptor antibody [HL1049] (GTX636021) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636021 IHC-P Image
Androgen Receptor antibody [HL1049] detects Androgen Receptor protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human prostate cancer.Androgen Receptor stained by Androgen Receptor antibody [HL1049] (GTX636021) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636021 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Androgen Receptor antibody [HL1049] (GTX636021) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636021 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Androgen Receptor antibody [HL1049] (GTX636021) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636021 ICC/IF Image
Androgen Receptor antibody [HL1049] detects Androgen Receptor protein at nucleus by immunofluorescent analysis.Sample: 22RV1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Androgen Receptor stained by Androgen Receptor antibody [HL1049] (GTX636021) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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GTX636021 IHC-P Image
Androgen Receptor antibody [HL1049] detects Androgen Receptor protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.Androgen Receptor stained by Androgen Receptor antibody [HL1049] (GTX636021) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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