APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.5μg/ml |
| 10μg/ml |
| 2-3μg/ml |
| Assay dependent |
|
Note :
ICC/IF
Strong expression of the protein seen in the mitochondrial/cytoplasmic of MCF7 and U2OS cells.
IHC-P
Human Prostate shows nuclear staining in the secretory cells of the gland.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX19066-PEP).
Product Note
This antibody is expected to recognize isoform 1 (NP_000035.2) only.
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence EVQLGLGRVYPRPPSC, from the N Terminus of the protein sequence according to NP_000035.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
androgen receptor , AIS , AR8 , DHTR , HUMARA , HYSP1 , KD , NR3C4 , SBMA , SMAX1 , TFM
Cellular Localization
Nucleus , Cytoplasm
Background
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]
Database
Research Area
DATA IMAGES
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GTX19066 ICC/IF Image
ICC/IF analysis of PFA fixed MCF7 cells using GTX19066 Androgen Receptor antibody, N-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
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GTX19066 WB Image
WB analysis of human cerebellum (A) and negative control pancreas (B) lysate using GTX19066 Androgen Receptor antibody, N-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
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GTX19066 ICC/IF Image
ICC/IF analysis of PFA fixed U2OS cells using GTX19066 Androgen Receptor antibody, N-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
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GTX19066 ChIP assay Image
ChIP analysis of DHT-treated HEC50 cells using GTX19066 Androgen Receptor antibody, N-term. The precipitated DNA was detected by PCR with primer set targeting to FKBP5.
ChIP reaction : 2μg antibody / 1 μg chromatin
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GTX19066 IHC-P Image
IHC-P analysis of human prostate using GTX19066 Androgen Receptor antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml
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REFERENCE
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REVIEW
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