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Androgen Receptor antibody [N1], N-term

Anti-Androgen Receptor antibody [N1], N-term used in Western Blot (WB). GTX100056
Anti-Androgen Receptor antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100056
Anti-Androgen Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100056
Anti-Androgen Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100056
Anti-Androgen Receptor antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100056

Cat. No. GTX100056

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Androgen Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Androgen Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240448

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

androgen receptor , AIS , AR8 , DHTR , HUMARA , HYSP1 , KD , NR3C4 , SBMA , SMAX1 , TFM

Cellular Localization

Nucleus

Background

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Androgen Receptor antibody [N1], N-term used in Western Blot (WB). GTX100056

GTX100056 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Androgen Receptor antibody [N1], N-term (GTX100056) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Androgen Receptor antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100056

GTX100056 ICC/IF Image

Androgen Receptor antibody [N1], N-term detects Androgen Receptor protein at nucleus by immunofluorescent analysis.
Sample: LNCap cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Androgen Receptor stained by Androgen Receptor antibody [N1], N-term (GTX100056) diluted at 1:2000.

Anti-Androgen Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100056

GTX100056 IHC-P Image

Androgen Receptor antibody [N1], N-term detects Androgen Receptor protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Androgen Receptor stained by Androgen Receptor antibody [N1], N-term (GTX100056) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Androgen Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100056

GTX100056 IHC-P Image

Androgen Receptor antibody [N1], N-term detects Androgen Receptor protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Androgen Receptor antibody [N1], N-term (GTX100056) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Androgen Receptor antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100056

GTX100056 ICC/IF Image

The data was published in the journal Oncotarget in 2020.PMID: 32256979

REFERENCE

Application Reference

Soave C et al. Biochem J 2022; 479 (14) : 1519-1531 Identification of ELK1 interacting peptide segments in the androgen receptor.
Assoun EN et al. Oncotarget 2020; 11 (12) : 1075-1084 Characterization of iPS87, a prostate cancer stem cell-like cell line.
Application : ICC/IF
Reactivity : Human
Yin et al. Biomedical Research 2017; (Epub) Altered expression of sex hormone receptors in keratoconus corneas.
Application : IHC-P
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169