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Androgen Receptor antibody [N1], N-term

Anti-Androgen Receptor antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100056
Anti-Androgen Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100056
Anti-Androgen Receptor antibody [N1], N-term used in Western Blot (WB). GTX100056

Cat No. GTX100056

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Positive Control

LNCaP

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Androgen Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

androgen receptor,AIS,AR8,DHTR,HUMARA,HYSP1,KD,NR3C4,SBMA,SMAX1,TFM

Cellular Localization

Nucleus

Background

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Androgen Receptor antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100056

GTX100056 ICC/IF Image

Androgen Receptor antibody [N1], N-term detects Androgen Receptor protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Androgen Receptor protein stained by Androgen Receptor antibody [N1], N-term (GTX100056) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Androgen Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100056

GTX100056 IHC-P Image

Androgen Receptor antibody [N1], N-term detects Androgen Receptor protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Androgen Receptor antibody [N1], N-term (GTX100056) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Androgen Receptor antibody [N1], N-term used in Western Blot (WB). GTX100056

GTX100056 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Androgen Receptor antibody [N1], N-term (GTX100056) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169