Angiogenin antibody [HL2214]

Cat. No. GTX638218

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL2214
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

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Datasheet

View product citations for antibody GTX638218 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Angiogenin.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

angiogenin , ALS9 , HEL168 , RAA1 , RNASE4 , RNASE5

Cellular Localization

Secreted , Nucleus , Nucleolus

Background

The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2014]

Database

Gene ID: 283 ANG
UniProt: P03950 ANG

Research Area

DATA IMAGES

GTX638218 WB Image

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Angiogenin antibody [HL2214] (GTX638218) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX638218 ICC/IF Image

Angiogenin antibody [HL2214] detects Angiogenin protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Angiogenin stained by Angiogenin antibody [HL2214] (GTX638218) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX638218 IHC-P Image

Angiogenin antibody [HL2214] detects Angiogenin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Angiogenin stained by Angiogenin antibody [HL2214] (GTX638218) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations