*Optimal dilutions/concentrations should be determined by the researcher.
|1:500 - 1:2000
|1:200 - 1:800
|1:5000 - 1:25000
Not tested in other applications.
This antibody is specific for mouse Angiopoietin 1 (mAng-1). Partially cross reactivity is noted with human angiopoietin 2.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
80 mg/ml (Please refer to the vial label for the specific concentration.)
Peptide: CNQRRNPENGGRRYNRIQHGQ (available as GTX29076)Region near the N-terminus of mouse Ang-1 (aa21-40).
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver. To our knowledge, this is the first time that an Ang antibody recognizes a band above 55 kD. This antibody (and the ang-2 antibody, GT28452) give a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher that the calculated molecular weight of 55 kD.