GeneTex
United States (US)

Angiopoietin 1 antibody

GTX28451_20160330_IHC-P_18121410_272.jpg
GTX28451_20160330_WB_18121410_431.jpg

Cat No. GTX28451

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:200 - 1:800
ELISA 1:5000 - 1:25000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Specificity/Sensitivity

This antibody is specific for mouse Angiopoietin 1 (mAng-1). Partially cross reactivity is noted with human angiopoietin 2.
PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

80 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Peptide: CNQRRNPENGGRRYNRIQHGQ (available as GTX29076)Region near the N-terminus of mouse Ang-1 (aa21-40).

Purification

Antiserum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Angiopoietin 1,1110046O21Rik,Ang-1,Ang1,Angpt1

Background

Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver. To our knowledge, this is the first time that an Ang antibody recognizes a band above 55 kD. This antibody (and the ang-2 antibody, GT28452) give a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher that the calculated molecular weight of 55 kD.

Database

Research Area

DATA IMAGES
GTX28451_20160330_IHC-P_18121410_272.jpg

GTX28451 IHC-P Image

IHC-P of ANG1 antibody was diluted 1:500 to detect ANG1 in human lung tissue. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

GTX28451_20160330_WB_18121410_431.jpg

GTX28451 WB Image

Rabbit anti-Ang-1 (GTX28451) was used at a 1:500 dilution to detect mouse Ang-1 by western blot against supernatants of mouse angiopoietin-expressing endothelial cells. Lane 1 - wt endothelial cells. Lane 2 - mouse Ang-1 (clone 1-8) expressing cells. Lane 3 - mouse Ang-1 (clone 1-15) expressing cells. Lane 4 - mouse Ang-2 (clone 2-9) expressing cells. Approximately 20 µg of each lysate was used for 10% SDS-PAGE.

REFERENCE