Angiopoietin 2 antibody [Angy-1-4] (Azide free)

Cat. No. GTX33914-00

Host	Human
Clonality	Monoclonal
Clone Name	Angy-1-4
Isotype	IgG2
Application	ELISA, Neutralizing/Inhibition
Reactivity	Human
Package	100 μg ($469)

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Datasheet
Document

View product citations for antibody GTX33914-00 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
ELISA	Assay dependent
Neutralizing/Inhibition	Assay dependent
Note : Neutralizing/Inhibition Inhibits the binding of human angiopoietin-2 to human Tie-2. ND50* = 600-800ng/ml (for 10ng/ml of Angiopoietin-2) *ND50: 50% neutralizing dose of antibody for a given concentration of ligand (here human Angiopoietin-2).

Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Product Note

Recognizes human angiopoietin-2. Does not recognize mouse angiopoietin-2 and human angiopoietin-1.

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human angiopoietin-2.

Purification

Protein A-affinity purified.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

angiopoietin 2 , AGPT2 , ANG2

Cellular Localization

Secreted

Background

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 285 ANGPT2
UniProt: O15123 ANGPT2

Research Area

DATA IMAGES

GTX33914-00 ELISA Image

Binding of human Angiopoietin-2 to Tie-2 (human):Fc is inhibited by the antibody anti-Angiopoietin-2 (human), mAb (rec.) (blocking) (Angy-1-4) (preservative free). Methods: Tie-2 (human):Fc was coated on an ELISA plate at 1μg/ml. anti-Angiopoietin-2 (human), mAb (rec.) (blocking), (Angy-1-4) (preservative free) or an unrelated mAb (recombinant) (Control) were added (starting at 40μg/ml with a twofold serial dilution) together with 200ng/μl of Angiopoietin-2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).

GTX33914-00 Image

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf

Package

List Price ($)

100 μg

$ 469

Qty