Binding of human Angiopoietin-2 to Tie-2 (human): Fc is inhibited by the antibody anti-Angiopoietin-2 (human), mAb (rec.) (blocking) (Angy-1-4). Methods: Tie-2 (human):Fc was coated on an ELISA plate at 1μg/ml. anti-Angiopoietin-2 (human), mAb (rec.) (blocking), (Angy-1-4) or an unrelated mAb (recombinant) (Control) were added (starting at 40μg/ml with a twofold serial dilution) together with 200ng/μl of Angiopoietin-2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).