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Angiopoietin 2 antibody [Angy-1-4]

Anti-Angiopoietin 2 antibody [Angy-1-4] used in  (). GTX33914
Anti-Angiopoietin 2 antibody [Angy-1-4] used in ELISA (ELISA). GTX33914

Cat No. GTX33914

Host Human
Clonality Monoclonal
Clone Name Angy-1-4
Isotype IgG2
Application ELISA, Functional Assay
Reactivity Human
APPLICATION

Application Note

Functional: Inhibits the binding of human angiopoietin-2 to human Tie-2. ND50 = 600-800ng/ml (for 10ng/ml of Angiopoietin-2) ND50: 50% neutralizing dose of antibody for a given concentration of ligand (here human Angiopoietin-2).

Calculated MW

57 kDa. ( Note )

Specificity/Sensitivity

Recognizes human angiopoietin-2. Does not recognize mouse angiopoietin-2 and human angiopoietin-1.
PROPERTIES

Form

Liquid

Buffer

PBS containing 10% glycerol and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human angiopoietin-2.

Purification

Protein A-affinity purified.

Purity

≧95% (SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Angiopoietin 2,Agpt2,Ang2,Angpt2

Background

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Angiopoietin 2 antibody [Angy-1-4] used in  (). GTX33914

GTX33914 Image

10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.

Anti-Angiopoietin 2 antibody [Angy-1-4] used in ELISA (ELISA). GTX33914

GTX33914 ELISA Image

Binding of human Angiopoietin-2 to Tie-2 (human): Fc is inhibited by the antibody anti-Angiopoietin-2 (human), mAb (rec.) (blocking) (Angy-1-4). Methods: Tie-2 (human):Fc was coated on an ELISA plate at 1µg/ml. anti-Angiopoietin-2 (human), mAb (rec.) (blocking), (Angy-1-4) or an unrelated mAb (recombinant) (Control) were added (starting at 40µg/ml with a twofold serial dilution) together with 200ng/µl of Angiopoietin-2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).