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Angiopoietin 2 antibody [Angy-2-1]

Anti-Angiopoietin 2 antibody [Angy-2-1] used in (). GTX33916
Anti-Angiopoietin 2 antibody [Angy-2-1] used in ELISA (ELISA). GTX33916

Cat. No. GTX33916

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 Angy-2-1

Isotype

 IgG2b

Application

 ELISA, Neutralizing/Inhibition

Reactivity

 Human, Mouse
Package
100 μg ($469)
See all Angiopoietin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ELISA Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

Neutralizing/Inhibition
Inhibits the binding of mouse angiopoietin-2 to mouse Tie-2. ND50* = 50-60ng/ml (for 10ng/ml of mouse Angiopoietin-2)
Inhibits the binding of human angiopoietin-2 to human Tie-2. ND50* = 8-12ng/ml (for 10ng/ml of human Angiopoietin-2)
*ND50: 50% neutralizing dose of antibody for a given concentration of ligand (here Angiopoietin-2).
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Product Note

Recognizes human and mouse angiopoietin-2. Does not detect human angiopoietin-1.

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human angiopoietin-2.

Purification

Protein A-affinity purified.

Purity

??95% by SDS-PAGE.

Conjugation

Unconjugated

RRID

AB_2887728

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

angiopoietin 2 , AGPT2 , ANG2

Cellular Localization

Secreted

Background

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Angiopoietin 2 antibody [Angy-2-1] used in (). GTX33916

GTX33916 Image

Anti-Angiopoietin 2 antibody [Angy-2-1] used in ELISA (ELISA). GTX33916

GTX33916 ELISA Image

Binding of human Angiopoietin-2 to Tie-2 (human):Fc is inhibited by the antibody anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1). Methods: Tie-2 (h):Fc was coated on an ELISA plate at 1μg/ml. anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) or an unrelated mAb (recombinant) (Control) were added (starting at 40μg/ml with a twofold serial dilution) together with 20ng/μl of Angiopoietin-2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).

REFERENCE

Application Reference

Zhong J et al. Phytomedicine 2020; 78 : 153302 Notoginsenoside R1 activates the Ang2/Tie2 pathway to promote angiogenesis.
Application : Neutralizing/Inhibition

Clone Reference

An YA et al. Elife 2017; 6. pii:e24071 Angiopoietin-2 in white adipose tissue improves metabolic homeostasis through enhanced angiogenesis.
Application : Neutralizing/Inhibition
Reactivity : Mouse
Yun JH et al. Cell Death Dis. 2016; 7:e2101 Angiopoietin 2 induces astrocyte apoptosis via αvβ5-integrin signaling in diabetic retinopathy.
Application : Neutralizing/Inhibition
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 469