GeneTex
United States (US)

Angiopoietin 2 antibody [Angy-2-1]

GTX33916_20160831_Structure_18121410_418.jpg
GTX33916_20160831_ELISA_18121410_940.jpg

Cat No. GTX33916

Host Human
Clonality Monoclonal
Clone Name Angy-2-1
Isotype IgG2b
Application ELISA, Functional Assay, Neut
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ELISA Assay dependent
Functional Assay Assay dependent
Neut Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Specificity/Sensitivity

Recognizes human and mouse angiopoietin-2. Does not detect human angiopoietin-1.
PROPERTIES

Form

Liquid

Buffer

PBS containing 10% glycerol and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant human angiopoietin-2.

Purification

Protein A-affinity purified.

Purity

≧95% (SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AGPT2 Antibody , ANG2 Antibody , angiopoietin 2 Antibody , ANGPT2 Antibody

Background

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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Database

Research Area

DATA IMAGES
GTX33916_20160831_Structure_18121410_418.jpg

GTX33916 Image

Structure of the recombinant antibody anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1). The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a mouse IgG2b Fc region.

GTX33916_20160831_ELISA_18121410_940.jpg

GTX33916 ELISA Image

Binding of human Angiopoietin-2 to Tie-2 (human):Fc is inhibited by the antibody anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1). Methods: Tie-2 (h):Fc was coated on an ELISA plate at 1µg/ml. anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) or an unrelated mAb (recombinant) (Control) were added (starting at 40µg/ml with a twofold serial dilution) together with 20ng/µl of Angiopoietin-2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).

Reference