Binding of human Angiopoietin-2 to Tie-2 (human):Fc is inhibited by the antibody anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1). Methods: Tie-2 (h):Fc was coated on an ELISA plate at 1μg/ml. anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) or an unrelated mAb (recombinant) (Control) were added (starting at 40μg/ml with a twofold serial dilution) together with 20ng/μl of Angiopoietin-2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).