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Angiopoietin 2 antibody [Angy-2-1]

Anti-Angiopoietin 2 antibody [Angy-2-1] used in  (). GTX33916
Anti-Angiopoietin 2 antibody [Angy-2-1] used in ELISA (ELISA). GTX33916

Cat No. GTX33916

Host

Human

Clonality

Monoclonal

Clone Name

Angy-2-1

Isotype

IgG2b

Application

ELISA, Functional Assay, Neutralizing/Blocking

Reactivity

Human, Mouse
Package
100 μg ($419)

APPLICATION

Application Note

Functional: Inhibits the binding of mouse angiopoietin-2 to mouse Tie-2. ND50* = 50-60ng/ml (for 10ng/ml of mouse Angiopoietin-2) inhibits the binding of human angiopoietin-2 to human Tie-2. ND50 = 8-12ng/ml (for 10ng/ml of human Angiopoietin-2) ND50: 50% neutralizing dose of antibody for a given concentration of ligand (here Angiopoietin-2).

Calculated MW

57 kDa. ( Note )

Specificity/Sensitivity

Recognizes human and mouse angiopoietin-2. Does not detect human angiopoietin-1.

PROPERTIES

Form

Liquid

Buffer

PBS containing 10% glycerol and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human angiopoietin-2.

Purification

Protein A-affinity purified.

Purity

≧95% (SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Angiopoietin 2 , Agpt2 , Ang2 , Angpt2

Background

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Angiopoietin 2 antibody [Angy-2-1] used in  (). GTX33916

GTX33916 Image

10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.

Anti-Angiopoietin 2 antibody [Angy-2-1] used in ELISA (ELISA). GTX33916

GTX33916 ELISA Image

Binding of human Angiopoietin-2 to Tie-2 (human):Fc is inhibited by the antibody anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1). Methods: Tie-2 (h):Fc was coated on an ELISA plate at 1μg/ml. anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) or an unrelated mAb (recombinant) (Control) were added (starting at 40μg/ml with a twofold serial dilution) together with 20ng/μl of Angiopoietin-2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).

REFERENCE

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Package List Price ($)
$ 419