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Angiopoietin 2 antibody

Anti-Angiopoietin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100991
Anti-Angiopoietin 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100991
Anti-Angiopoietin 2 antibody used in Western Blot (WB). GTX100991
Anti-Angiopoietin 2 antibody used in Western Blot (WB). GTX100991
Anti-Angiopoietin 2 antibody used in Western Blot (WB). GTX100991

Cat No. GTX100991

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

HepG2 , HUVEC , mouse lung

Predict Reactivity

Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Angiopoietin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Angiopoietin 2,Agpt2,Ang2,Angpt2

Cellular Localization

Secreted

Background

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Angiopoietin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100991

GTX100991 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Angiopoietin 2(GTX100991) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Angiopoietin 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100991

GTX100991 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using Angiopoietin 2(GTX100991) antibody at 1:500 dilution.

Anti-Angiopoietin 2 antibody used in Western Blot (WB). GTX100991

GTX100991 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiopoietin 2 antibody (GTX100991) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Angiopoietin 2 antibody used in Western Blot (WB). GTX100991

GTX100991 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Angiopoietin 2 antibody (GTX100991) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Angiopoietin 2 antibody used in Western Blot (WB). GTX100991

GTX100991 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiopoietin 2 antibody (GTX100991) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169