GeneTex
United States (US)

Angiopoietin 2 antibody

Cat No. GTX10601

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.2 μg/mL
IHC-P 5-15 μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 5% trehalose

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant human angiopoietin-2 expressed in mouse NSO cells.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Angiopoietin 2,Agpt2,Ang2,Angpt2

Background

Angiopoietin 2 and angiopoietin 1 have an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain. The coiled coil domains mediate ligand homo-oligomerization and the fibrinogen-like domains mediate ligand activity. Recombinant human angiopoietin 2 has a molecular mass of approximately 66 kDa in SDS-PAGE under reducing and non-reducing conditions. Human and mouse angiopoietin 2 share 85% sequence identity and are 60% identical to their Ang1 homologs. Angiopoietin 2 and angiopoietin 1 are closely related endothelial cell-specific ligands of the endothelial cellspecific receptor Tie2 expressed on endothelial cells and early hematopoietic cells. Ang1, Ang2, and their receptor Tie2 play critical roles in embryogenic angiogenesis, adult vasculogenesis, and endothelial cell proliferation. Angiopoietin2, released by endothelial cells of interior tumor vessels, promotes vessel regression and disassociation from surrounding tumor cells. Both Ang1 and Ang2 induce phosphorylation of expressed receptors on NIH3T3 fibroblasts stably expressing transfected Tie2. However, only Ang1 induces a chemotactic response and Tie2 phosphorylation on endothelial cells. Ang2 inhibits Ang1 induced endothelial cell responses mediated by Tie2. Angiopoietin 2 probably acts as a natural antagonist for Ang1 and Tie2 both in vivo and in vitro. In adult mice and humans, angiopoietin 2 is expressed at sites of vascular remodeling.

Database

Research Area