GeneTex
United States (US)

Angiopoietin 2 antibody

GTX28452_20160330_IHC-P_18121410_876.jpg
GTX28452_20160330_WB_18121410_900.jpg

Cat No. GTX28452

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:200 - 1:500
ELISA 1:5000 - 1:25000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Specificity/Sensitivity

This antibody is specific for mouse Angiopoietin 2 (mAng-2). Partially cross-reactivity is noted with human angiopoietin-1
PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.1% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

80 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, corresponding to a region near the N-terminus of mouse angiopoietin-2 protein, conjugated to KLH using maleimide.

Purification

Antiserum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Angiopoietin 2,Agpt2,Ang-2,Ang2,Angpt2

Background

Angiopoietin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-2 expression occurs at sites of vascular remodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus. To our knowledge, this is the first time that an Ang antibody recognizes a band above 55 kD. This antibody (and the ang-1 antibody, GT28451) give a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher that the calculated molecular weight of 55 kD.

Database

Research Area

DATA IMAGES
GTX28452_20160330_IHC-P_18121410_876.jpg

GTX28452 IHC-P Image

IHC-P of anti-ANG2 Antibody (GTX28452) in human placenta tissue. Antigen Retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 2ug/ml for 1.5 hour RT.

GTX28452_20160330_WB_18121410_900.jpg

GTX28452 WB Image

Western blotting. Rabbit anti-Ang-2 was used at a 1:500 dilution to detect mouse Ang-2 in supernatants of mouse-angiopoietin-expressing endothelial cells. Lane 1 : mock endothelial cells. Lane 2 : mouse Ang-2 expressing cells. Lane 3 - mouse Ang-1 expressing cells. Lane 4 - mouse Ang-1 expressing cells. Lane 5 - wt endothelial cells. Approximately 20 µg of each lysate was loaded for 10% SDS-PAGE.

REFERENCE