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Angiopoietin 4 antibody

Anti-Angiopoietin 4 antibody used in Western Blot (WB). GTX100993
Anti-Angiopoietin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX100993
Anti-Angiopoietin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX100993

Cat. No. GTX100993

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Angiopoietin 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

H1299 , HepG2 , Molt-4

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Angiopoietin 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949650

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

angiopoietin 4 , ANG3 , ANG4

Cellular Localization

Secreted

Background

Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The mechanism by which they contribute to angiogenesis is thought to involve regulation of endothelial cell interactions with supporting perivascular cells. The protein encoded by this gene functions as an agonist and is an angiopoietin. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Angiopoietin 4 antibody used in Western Blot (WB). GTX100993

GTX100993 WB Image

Sample (30μg whole cell lysate)
A:H1299
B:Hep G2 (GTX27900)
C:MOLT4 (GTX27912)
7.5% SDS PAGE
GTX100993 diluted at 1:500

Anti-Angiopoietin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX100993

GTX100993 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DU145 xenograft, using Angiopoietin 4(GTX100993) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Angiopoietin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX100993

GTX100993 IHC-P Image

Angiopoietin 4 antibody detects Angiopoietin 4 protein at cytoplasm on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Angiopoietin 4 antibody (GTX100993) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169