GeneTex
United States (US)

Angiopoietin 4 antibody

GTX100993_39995_IHC_19011506_821.jpg
GTX100993_WB_19011506_330.jpg
GTX100993_39995_20150410_IHC_M_19011506_867.jpg

Cat No. GTX100993

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Angiopoietin 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Angiopoietin 4,Ang3,Ang4,Angpt4

Cellular Localization

Secreted

Background

Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The mechanism by which they contribute to angiogenesis is thought to involve regulation of endothelial cell interactions with supporting perivascular cells. The protein encoded by this gene functions as an agonist and is an angiopoietin. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100993_39995_IHC_19011506_821.jpg

GTX100993 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DU145 xenograft, using Angiopoietin 4(GTX100993) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100993_WB_19011506_330.jpg

GTX100993 WB Image

Sample (30μg whole cell lysate)
A:H1299
B:Hep G2 (GTX27900)
C:MOLT4 (GTX27912)
7.5% SDS PAGE
GTX100993 diluted at 1:500

GTX100993_39995_20150410_IHC_M_19011506_867.jpg

GTX100993 IHC-P Image

Angiopoietin 4 antibody detects Angiopoietin 4 protein at cytoplasm on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Angiopoietin 4 antibody (GTX100993) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169