Immunofluorescence analysis of methanol-fixed HepG2, using Angiotensinogen(GTX103824) antibody at 1:200 dilution.

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiotensinogen antibody [N1C3] (GTX103824) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiotensinogen antibody [N1C3] (GTX103824) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma Huh7 xenograft, using AGT(GTX103824) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiotensinogen antibody [N1C3] (GTX103824) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample(30 μg whole cell lysate)
A:Hep G2 (GTX27900)
10% SDS PAGE
GTX103824 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the 2022 in Front Physiol. PMID: 35222071