GeneTex
United States (US)

Angiotensinogen antibody [N1C3]

GTX103824_39946_IFA_19011506_111.jpg
GTX103824_IHC_19011506_793.jpg
GTX103824_39946_20160107_WB_R_plasma_19011506_817.jpg
GTX103824_39946_20160107_WB_H_plasma_19011506_826.jpg
GTX103824_WB_19011506_541.jpg
GTX103824_39946_20160107_WB_M_plasma_19011506_276.jpg

Cat No. GTX103824

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

HepG2 , human plasma , mouse plasma , rat plasma

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Angiotensinogen. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Angiotensinogen,Anhu,Serpina8,Hflt1,Agt

Background

The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103824_39946_IFA_19011506_111.jpg

GTX103824 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HepG2, using Angiotensinogen(GTX103824) antibody at 1:200 dilution.

GTX103824_IHC_19011506_793.jpg

GTX103824 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma Huh7 xenograft, using AGT(GTX103824) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103824_39946_20160107_WB_R_plasma_19011506_817.jpg

GTX103824 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiotensinogen antibody [N1C3] (GTX103824) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103824_39946_20160107_WB_H_plasma_19011506_826.jpg

GTX103824 WB Image

Human tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiotensinogen antibody [N1C3] (GTX103824) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103824_WB_19011506_541.jpg

GTX103824 WB Image

Sample(30 µg whole cell lysate)
A:Hep G2 (GTX27900)
10% SDS PAGE
GTX103824 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103824_39946_20160107_WB_M_plasma_19011506_276.jpg

GTX103824 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiotensinogen antibody [N1C3] (GTX103824) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169