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Angiotensinogen antibody [N1C3]

Orthogonal
Anti-Angiotensinogen antibody [N1C3] used in Western Blot (WB). GTX103824
Anti-Angiotensinogen antibody [N1C3] used in Western Blot (WB). GTX103824
Anti-Angiotensinogen antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103824
Anti-Angiotensinogen antibody [N1C3] used in Western Blot (WB). GTX103824
Anti-Angiotensinogen antibody [N1C3] used in Western Blot (WB). GTX103824
Anti-Angiotensinogen antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103824
Anti-Angiotensinogen antibody [N1C3] used in Western Blot (WB). GTX103824

Cat. No. GTX103824

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Angiotensinogen products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

HepG2 , human plasma , mouse plasma , rat plasma

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Angiotensinogen. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949605

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

angiotensinogen , ANHU , SERPINA8 , hFLT1

Background

The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Angiotensinogen antibody [N1C3] used in Western Blot (WB). GTX103824

GTX103824 WB Image

Sample(30 μg whole cell lysate)
A:Hep G2 (GTX27900)
10% SDS PAGE
GTX103824 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Angiotensinogen antibody [N1C3] used in Western Blot (WB). GTX103824

GTX103824 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiotensinogen antibody [N1C3] (GTX103824) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Angiotensinogen antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103824

GTX103824 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma Huh7 xenograft, using AGT(GTX103824) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Angiotensinogen antibody [N1C3] used in Western Blot (WB). GTX103824

GTX103824 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiotensinogen antibody [N1C3] (GTX103824) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Angiotensinogen antibody [N1C3] used in Western Blot (WB). GTX103824

GTX103824 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiotensinogen antibody [N1C3] (GTX103824) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Angiotensinogen antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103824

GTX103824 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HepG2, using Angiotensinogen(GTX103824) antibody at 1:200 dilution.

Anti-Angiotensinogen antibody [N1C3] used in Western Blot (WB). GTX103824

GTX103824 WB Image

Orthogonal

The data was published in the 2022 in Front Physiol. PMID: 35222071

REFERENCE

Application Reference

Aihua Lu et al. Front Physiol 2022; 13 : 784521 (Pro)renin Receptor Regulates Phosphate Homeostasis in Rats via Releasing Fibroblast Growth Factor-23.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169