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Anillin antibody [C3], C-term

Orthogonal
Anti-Anillin antibody [C3], C-term used in Western Blot (WB). GTX107742
Anti-Anillin antibody [C3], C-term used in Western Blot (WB). GTX107742
Anti-Anillin antibody [C3], C-term used in Western Blot (WB). GTX107742
Anti-Anillin antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107742
Anti-Anillin antibody [C3], C-term used in Western Blot (WB). GTX107742
Anti-Anillin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107742

Cat. No. GTX107742

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Anillin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , C8D30

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Anillin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240452

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

anillin actin binding protein , FSGS8 , Scraps , scra

Cellular Localization

Nucleus , Cytoplasm , cytoskeleton , cell cortex

Background

Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.

Database

Research Area

DATA IMAGES

Anti-Anillin antibody [C3], C-term used in Western Blot (WB). GTX107742

GTX107742 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Anillin antibody [C3], C-term (GTX107742) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Anillin antibody [C3], C-term used in Western Blot (WB). GTX107742

GTX107742 WB Image

U251 whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Anillin antibody [C3], C-term (GTX107742) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Anillin antibody [C3], C-term used in Western Blot (WB). GTX107742

GTX107742 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Anillin antibody [C3], C-term (GTX107742) diluted at 1:500.

Anti-Anillin antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107742

GTX107742 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Anillin(GTX107742) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Anillin antibody [C3], C-term used in Western Blot (WB). GTX107742

GTX107742 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Anillin antibody [C3], C-term (GTX107742) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

Anti-Anillin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107742

GTX107742 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human Kidney, using Anillin(GTX107742) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Lisi Luo et al. Am J Cancer Res 2021; 11 (2) : 495-512 A new 7-gene survival score assay for pancreatic cancer patient prognosis prediction.
Application : WB, IHC-P
Reactivity : Human
Maurin J et al. Eur J Cell Biol 2018; 97 (8) : 568-579 Combined strategy of siRNA and osteoclast actin cytoskeleton automated imaging to identify novel regulators of bone resorption shows a non-mitotic function for anillin.
Application : ICC/IF
Reactivity : Mouse
Pei YF et al. Biochim Biophys Acta 2017; (Epub) TOP2A induces malignant character of pancreatic cancer through activating β-catenin signaling pathway.
Application : WB
Reactivity : Human
Chang YW et al. Cell Cycle 2017; : 0 Midbody localization of Vinexin recruits Rhotekin to facilitate cytokinetic abscission.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169