GeneTex
United States (US)

Anillin antibody [C3], C-term

GTX107742_39721_IFA_19011507_991.jpg
GTX107742_39721_20170309_WB_M_19011507_848.jpg
GTX107742_39721_IHC_19011507_514.jpg
GTX107742_39721_20170309_WB_19011507_168.jpg

Cat No. GTX107742

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , C8D30
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Anillin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Anillin Actin Binding Protein,Fsgs8,Scraps,Scra,Anln

Cellular Localization

Nucleus , Cytoplasm , cytoskeleton , cell cortex

Background

Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.

Database

Research Area

DATA IMAGES
GTX107742_39721_IFA_19011507_991.jpg

GTX107742 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Anillin(GTX107742) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX107742_39721_20170309_WB_M_19011507_848.jpg

GTX107742 WB Image

Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Anillin antibody [C3], C-term (GTX107742) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

GTX107742_39721_IHC_19011507_514.jpg

GTX107742 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human Kidney, using Anillin(GTX107742) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107742_39721_20170309_WB_19011507_168.jpg

GTX107742 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Anillin antibody [C3], C-term (GTX107742) diluted at 1:500.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169