GeneTex
United States (US)

Annexin A1 antibody

KO/KD Overexpression
GTX101070_40352_20190201_WB_KO_watermark_19022001_498.jpg
GTX101070_40150_WB_M_19011506_520.jpg
GTX101070_40150_20180727_WB_B_19011506_671.jpg
GTX101070_40150_20150731_IP_19011506_844.jpg
GTX101070_40150_IHC_M_19011506_755.jpg
GTX101070_40352_WB_19011506_953.jpg
GTX101070_IHC_19011506_457.jpg
GTX101070_IFA_19011506_956.jpg

Cat No. GTX101070

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

HeLa , NIH-3T3 , JC , ANXA1-transfected 293T

Predict Reactivity

Rabbit, Bovine, Dog, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Annexin A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Annexin A1,Anx1,Lpc1,Anxa1

Cellular Localization

Nucleus , Cytoplasm , Cell projection , cilium , Basolateral cell membrane

Background

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101070_40352_20190201_WB_KO_watermark_19022001_498.jpg

GTX101070 WB Image

KO/KD

Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101070_40150_WB_M_19011506_520.jpg

GTX101070 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
B: JC
10% SDS PAGE
GTX101070 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101070_40150_20180727_WB_B_19011506_671.jpg

GTX101070 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101070_40150_20150731_IP_19011506_844.jpg

GTX101070 IP Image

Immunoprecipitation of Annexin A1 protein from HeLa whole cell extracts using 5 μg of Annexin A1 antibody (GTX101070).
Western blot analysis was performed using Annexin A1 antibody (GTX101070).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101070_40150_IHC_M_19011506_755.jpg

GTX101070 IHC-P Image

Annexin A1 antibody detects Annexin A1 protein at cytosol and nucleus on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Annexin A1 antibody (GTX101070) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101070_40352_WB_19011506_953.jpg

GTX101070 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX101070 diluted at 1:10000

GTX101070_IHC_19011506_457.jpg

GTX101070 IHC-P Image

Immunohistochemical analysis of paraffin-embedded N87 Xenograft , using Annexin I(GTX101070) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101070_IFA_19011506_956.jpg

GTX101070 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Annexin I(GTX101070) antibody at 1:200 dilution.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169