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Annexin A1 antibody

KO/KD Comparison
Anti-Annexin A1 antibody used in Western Blot (WB). GTX113329
Anti-Annexin A1 antibody used in Western Blot (WB). GTX113329
Anti-Annexin A1 antibody used in Western Blot (WB). GTX113329
Anti-Annexin A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113329
Anti-Annexin A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113329
Anti-Annexin A1 antibody used in Western Blot (WB). GTX113329

Cat. No. GTX113329

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Annexin A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

NIH-3T3 , H1299 , HeLa

Predict Reactivity

Rat, Rabbit, Bovine, Cat, Dog, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Annexin A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036235

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

annexin A1 , ANX1 , LPC1

Cellular Localization

Nucleus , Cytoplasm , Cell projection , cilium , Basolateral cell membrane

Background

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Annexin A1 antibody used in Western Blot (WB). GTX113329

GTX113329 WB Image

KO/KD

Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX113329) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin A1 antibody used in Western Blot (WB). GTX113329

GTX113329 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Annexin A1 antibody (GTX113329) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Annexin A1 antibody used in Western Blot (WB). GTX113329

GTX113329 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX113329 diluted at 1:10000

Anti-Annexin A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113329

GTX113329 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Annexin A1(GTX113329) antibody at 1:200 dilution.

Anti-Annexin A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113329

GTX113329 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Annexin A1(GTX113329) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Annexin A1 antibody used in Western Blot (WB). GTX113329

GTX113329 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX113329 diluted at 1:1000

REFERENCE

Application Reference

Alhasan H et al. Nat Commun 2022; 13 (1) : 3919 Inhibitory role of Annexin A1 in pathological bone resorption and therapeutic implications in periprosthetic osteolysis.
Application : IHC-P
Reactivity : Human
Chang SJ et al. Arch Biochem Biophys 2018; 647 : 10-32 Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.
Application : WB
Reactivity : Human
Gueugneau M et al. BMC Genomics 2014; 15 (1) : 1165 Proteomics of muscle chronological ageing in post-menopausal women.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169