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Annexin II antibody [N1], N-term

KO/KD Overexpression
GTX100046_40240_20150730_WB_B_19011506_386.jpg
GTX100046_40240_20170112_WB_KO_watermark_19011506_923.jpg
GTX100046_IHC2_19011506_856.jpg
GTX100046_IHC1_19011506_123.jpg
GTX100046_40240_WB_19011506_992.jpg
GTX100046_39476_IFA_19011506_196.jpg

Cat No. GTX100046

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

A549 , HeLa , HCT116 , human ANXA2-transfected 293T

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Annexin II. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ANX2 antibody, ANX2L4 antibody, CAL1H antibody, LIP2 antibody, LPC2 antibody, LPC2D antibody, P36 antibody, PAP-IV antibody, ANXA2 antibody, placental anticoagulant protein IV antibody, calpactin I heavy polypeptide antibody, annexin II antibody, lipocortin II antibody, calpactin-1 heavy chain antibody, chromobindin 8 antibody, chromobindin-8 antibody, annexin-2 antibody, annexin A2 antibody, calpactin I heavy chain antibody, protein I antibody

Cellular Localization

Secreted , extracellular space , extracellular matrix , basement membrane , Melanosome

Background

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100046_40240_20150730_WB_B_19011506_386.jpg

GTX100046 WB Image

Overexpression

Annexin II antibody detects Annexin II protein by western blot analysis. Non-transfected (-) and human Annexin I or Annexin II-transfected (+) whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody (GTX100046) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100046_40240_20170112_WB_KO_watermark_19011506_923.jpg

GTX100046 WB Image

KO/KD

Wild-type (WT) and Annexin II knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody [N1], N-term (GTX100046) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX100046_IHC2_19011506_856.jpg

GTX100046 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue, using ANXA2(GTX100046) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100046_IHC1_19011506_123.jpg

GTX100046 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue, using ANXA2(GTX100046) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100046_40240_WB_19011506_992.jpg

GTX100046 WB Image

Annexin II antibody [N1], N-term detects ANXA2 protein by western blot analysis.
A. 30 µg A549 whole cell lysate/extract
B. 30 µg HCT116 whole cell lysate/extract
10% SDS-PAGE
Annexin II antibody [N1], N-term (GTX100046) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100046_39476_IFA_19011506_196.jpg

GTX100046 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Annexin II(GTX100046) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169