APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
A549 , HCT116 , human ANXA2-transfected 293T , HeLa
Predict Reactivity
Mouse, Rat, Sheep, Bovine, Dog, Chicken, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.75 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Annexin II. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240455
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
annexin A2 , ANX2 , ANX2L4 , CAL1H , HEL-S-270 , LIP2 , LPC2 , LPC2D , P36 , PAP-IV
Cellular Localization
Secreted , extracellular space , extracellular matrix , basement membrane , Melanosome
Background
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX100046 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Annexin II(GTX100046) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
|
|
GTX100046 WB Image
Wild-type (WT) and Annexin II knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody [N1], N-term (GTX100046) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX100046 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue, using ANXA2(GTX100046) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
GTX100046 WB Image
Annexin II antibody detects Annexin II protein by western blot analysis. Non-transfected (-) and human Annexin I or Annexin II-transfected (+) whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody (GTX100046) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX100046 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue, using ANXA2(GTX100046) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
GTX100046 WB Image
Annexin II antibody [N1], N-term detects ANXA2 protein by western blot analysis. A. 30 μg A549 whole cell lysate/extract B. 30 μg HCT116 whole cell lysate/extract 10% SDS-PAGE Annexin II antibody [N1], N-term (GTX100046) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
REVIEW
There are currently no reviews for Annexin II antibody [N1], N-term (GTX100046). Be the first to share your experience with this product.