GeneTex
United States (US)

Annexin II antibody

KO/KD Overexpression
GTX101902_39764_20170112_WB_KO_watermark_19011506_781.jpg
GTX101902_IFA_19011506_731.jpg
GTX101902_39764_IHC_19011506_750.jpg
GTX101902_39764_20150730_WB_B_19011506_214.jpg
GTX101902_39764_WB_R_19011506_863.jpg
GTX101902_39764_WB_19011506_476.jpg
GTX101902_39764_WB_M_19011506_908.jpg

Cat No. GTX101902

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

A549 , HCT116 , HeLa , GL261 , NIH-3T3 , C2C12 , Rat2 , human ANXA2-transfected 293T

Predict Reactivity

Sheep, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Annexin II. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Annexin A2,Anx2,Anx2L4,Cal1H,Hel-S-270,Lip2,Lpc2,Lpc2D,P36,Pap-Iv,Anxa2

Cellular Localization

Secreted , extracellular space , extracellular matrix , basement membrane , Melanosome

Background

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101902_39764_20170112_WB_KO_watermark_19011506_781.jpg

GTX101902 WB Image

KO/KD

Wild-type (WT) and Annexin II knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody (GTX101902) diluted at 1:1000.
CRISPR knockout cell lines generated by www.edigene.com

GTX101902_IFA_19011506_731.jpg

GTX101902 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Annexin II (GTX101902) antibody at 1:200 dilution.

GTX101902_39764_IHC_19011506_750.jpg

GTX101902 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Annexin II antibody(GTX101902) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101902_39764_20150730_WB_B_19011506_214.jpg

GTX101902 WB Image

Overexpression

Annexin II antibody detects Annexin II protein by western blot analysis. Non-transfected (-) and human Annexin I or Annexin II-transfected (+) whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody (GTX101902) diluted at 1:1000.

GTX101902_39764_WB_R_19011506_863.jpg

GTX101902 WB Image

Annexin II antibody detects ANXA2 protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
10 % SDS-PAGE
Annexin II antibody (GTX101902) dilution: 1:1000

GTX101902_39764_WB_19011506_476.jpg

GTX101902 WB Image

Annexin II antibody detects ANXA2 protein by Western blot analysis.
A. 30 µg A549 whole cell lysate/extract
B. 30 µg HCT116 whole cell lysate/extract
10 % SDS-PAGE
Annexin II antibody (GTX101902) dilution: 1:1000

GTX101902_39764_WB_M_19011506_908.jpg

GTX101902 WB Image

Annexin II antibody detects ANXA2 protein by Western blot analysis.
A. 30 µg GL261 whole cell lysate/extract
B. 30 µg NIH-3T3 whole cell lysate/extract
C. 30 µg C2C12 whole cell lysate/extract
10 % SDS-PAGE
Annexin II antibody (GTX101902) dilution: 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169