GeneTex

  • Country / Location Selection

United States (US)

Annexin II antibody

KO/KD Overexpression
Anti-Annexin II antibody used in Western Blot (WB). GTX101902
Anti-Annexin II antibody used in Western Blot (WB). GTX101902
Anti-Annexin II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101902
Anti-Annexin II antibody used in Western Blot (WB). GTX101902
Anti-Annexin II antibody used in Western Blot (WB). GTX101902
Anti-Annexin II antibody used in IHC (Paraffin sections) (IHC-P). GTX101902
Anti-Annexin II antibody used in Western Blot (WB). GTX101902
Anti-Annexin II antibody used in Western Blot (WB). GTX101902

Cat. No. GTX101902

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Annexin II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

GL261 , NIH-3T3 , C2C12 , Rat2

Predict Reactivity

Sheep, Bovine, Dog, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Annexin II. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949658

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

annexin A2 , ANX2 , ANX2L4 , CAL1H , HEL-S-270 , LIP2 , LPC2 , LPC2D , P36 , PAP-IV

Cellular Localization

Secreted , extracellular space , extracellular matrix , basement membrane , Melanosome

Background

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Annexin II antibody used in Western Blot (WB). GTX101902

GTX101902 WB Image

KO/KD

Wild-type (WT) and Annexin II knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody (GTX101902) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin II antibody used in Western Blot (WB). GTX101902

GTX101902 WB Image

Annexin II antibody detects ANXA2 protein by Western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
Annexin II antibody (GTX101902) dilution: 1:1000

Anti-Annexin II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101902

GTX101902 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Annexin II (GTX101902) antibody at 1:200 dilution.

Anti-Annexin II antibody used in Western Blot (WB). GTX101902

GTX101902 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody (GTX101902) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin II antibody used in Western Blot (WB). GTX101902

GTX101902 WB Image

Annexin II antibody detects ANXA2 protein by Western blot analysis.
A. 30 μg GL261 whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
C. 30 μg C2C12 whole cell lysate/extract
10 % SDS-PAGE
Annexin II antibody (GTX101902) dilution: 1:1000

Anti-Annexin II antibody used in IHC (Paraffin sections) (IHC-P). GTX101902

GTX101902 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Annexin II antibody(GTX101902) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Annexin II antibody used in Western Blot (WB). GTX101902

GTX101902 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody (GTX101902) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin II antibody used in Western Blot (WB). GTX101902

GTX101902 WB Image

The data was published in the journal Breast Cancer Res in 2014. PMID: 24512624

REFERENCE

Application Reference

Linn Streubel-Gallasch et al. Mol Neurobiol 2021; 58 (7) : 3119-3140 Parkinson's Disease-Associated LRRK2 Interferes with Astrocyte-Mediated Alpha-Synuclein Clearance.
Application : WB, ICC/IF
Reactivity : Human, Mouse
Kawakatsu K et al. Anticancer Res 2021; 41 (8) : 4089-4092 Involvement of Annexin A2 in Serum-MAF Dependent Phagocytic Activation of Macrophages.
Fan SM et al. Biomaterials 2018; 167 : 121-131 Inducing hair follicle neogenesis with secreted proteins enriched in embryonic skin.
Kowal J et al. Proc Natl Acad Sci U S A 2016; 113 (8) : E968-77 Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes.
Application : WB
Reactivity : Human
Gangalum RK et al. J Biol Chem 2016; (Epub) Inhibition of the Expression of the Small Heat Shock Protein αB-crystallin Inhibits Exosome Secretion in Human Retinal Pigment Epithelial Cells in Culture.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

There are currently no reviews for Annexin II antibody (GTX101902). Be the first to share your experience with this product.
Submit a Review
Application Reference
Linn Streubel-Gallasch et al. Mol Neurobiol 2021; 58 (7):3119-3140 Parkinson's Disease-Associated LRRK2 Interferes with Astrocyte-Mediated Alpha-Synuclein Clearance.
Application : WB, ICC/IF
Reactivity : Human, Mouse
Kawakatsu K et al. Anticancer Res 2021; 41 (8):4089-4092 Involvement of Annexin A2 in Serum-MAF Dependent Phagocytic Activation of Macrophages.
Fan SM et al. Biomaterials 2018; 167:121-131 Inducing hair follicle neogenesis with secreted proteins enriched in embryonic skin.
Kowal J et al. Proc Natl Acad Sci U S A 2016; 113 (8):E968-77 Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes.
Application : WB
Reactivity : Human
Gangalum RK et al. J Biol Chem 2016; (Epub) Inhibition of the Expression of the Small Heat Shock Protein αB-crystallin Inhibits Exosome Secretion in Human Retinal Pigment Epithelial Cells in Culture.
Application : WB
Reactivity : Human
Chao PZ et al. Oncotarget 2015; 6 (1):159-70 Dendritic cells respond to nasopharygeal carcinoma cells through annexin A2-recognizing DC-SIGN.
Reactivity : Mouse
Babina IS et al. Breast Cancer Res 2014; 16 (1):R19 A novel mechanism of regulating breast cancer cell migration via palmitoylation-dependent alterations in the lipid raft affiliation of CD44.
Application : WB
Reactivity : Human
Bulloj A et al. Exp Eye Res 2013; 113:43361 PI 3-kinase independent role for AKT in F-actin regulation during outer segment phagocytosis by RPE cells.
Application : ICC/IF
Reactivity : Rat
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169