GeneTex
  • Country / Location Selection

United States (US)

Annexin III antibody

Anti-Annexin III antibody used in Western Blot (WB). GTX103330
Anti-Annexin III antibody used in IHC (Paraffin sections) (IHC-P). GTX103330

Cat No. GTX103330

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

A549 , HCT116

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Annexin III. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

annexin A3 , ANX3

Background

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Annexin III antibody used in Western Blot (WB). GTX103330

GTX103330 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: HCT116
10% SDS PAGE
GTX103330 diluted at 1:10000

Anti-Annexin III antibody used in IHC (Paraffin sections) (IHC-P). GTX103330

GTX103330 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Annexin III(GTX103330) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

Annexin III antibody Cat No. GTX103330

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 25-Aug-2016
Score :
Application Tested : WB
Sample Species : Rat
Sample : Rat brain tissue
Amount used : 25μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169