GeneTex

  • Country / Location Selection

United States (US)

Annexin IV antibody

Anti-Annexin IV antibody used in Western Blot (WB). GTX103249
Anti-Annexin IV antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103249
Anti-Annexin IV antibody used in IHC (Paraffin sections) (IHC-P). GTX103249
Anti-Annexin IV antibody used in Immunoprecipitation (IP). GTX103249

Cat. No. GTX103249

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Annexin IV products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Annexin IV. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617502

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

annexin A4 , ANX4 , HEL-S-274 , P32.5 , PAP-II , PIG28 , PP4-X , ZAP36

Background

Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Annexin IV antibody used in Western Blot (WB). GTX103249

GTX103249 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
Annexin IV antibody
GTX103249 diluted at 1:1000

Anti-Annexin IV antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103249

GTX103249 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Annexin A4(GTX103249) antibody at 1:200 dilution.

Anti-Annexin IV antibody used in IHC (Paraffin sections) (IHC-P). GTX103249

GTX103249 IHC-P Image

Annexin IV antibody detects ANXA4 protein at cytosol and membrane on D54MG xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded D54MG xenograft.
Annexin IV antibody (GTX103249) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Annexin IV antibody used in Immunoprecipitation (IP). GTX103249

GTX103249 IP Image

Immunoprecipitation of Annexin IV protein from HepG2 whole cell extracts using 5 μg of Annexin IV antibody (GTX103249).
Western blot analysis was performed using Annexin IV antibody (GTX103249).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

Application Reference

Chen JF et al. J Pharm Biomed Anal 2020; 187 : 113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Annexin IV antibody (GTX103249). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169