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Annexin IV antibody

Anti-Annexin IV antibody used in Immunoprecipitation (IP). GTX103249
Anti-Annexin IV antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103249
Anti-Annexin IV antibody used in Western Blot (WB). GTX103249
Anti-Annexin IV antibody used in IHC (Paraffin sections) (IHC-P). GTX103249

Cat No. GTX103249

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Annexin IV. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

annexin A4 , ANX4 , HEL-S-274 , P32.5 , PAP-II , PIG28 , PP4-X , ZAP36

Background

Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Annexin IV antibody used in Immunoprecipitation (IP). GTX103249

GTX103249 IP Image

Immunoprecipitation of Annexin IV protein from HepG2 whole cell extracts using 5 μg of Annexin IV antibody (GTX103249).
Western blot analysis was performed using Annexin IV antibody (GTX103249).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Annexin IV antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103249

GTX103249 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Annexin A4(GTX103249) antibody at 1:200 dilution.

Anti-Annexin IV antibody used in Western Blot (WB). GTX103249

GTX103249 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
Annexin IV antibody
GTX103249 diluted at 1:1000

Anti-Annexin IV antibody used in IHC (Paraffin sections) (IHC-P). GTX103249

GTX103249 IHC-P Image

Annexin IV antibody detects ANXA4 protein at cytosol and membrane on D54MG xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded D54MG xenograft.
Annexin IV antibody (GTX103249) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169