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Annexin V-EGFP Apoptosis Detection Kit

Annexin V-EGFP Apoptosis Detection Kit. GTX85588
Annexin V-EGFP Apoptosis Detection Kit. GTX85588
Annexin V-EGFP Apoptosis Detection Kit. GTX85588

Cat No. GTX85588

Application ICC/IF
Package
100 assay ($439)
PRODUCT

Product Description

The Annexin V-EGFP Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with an enhanced green fluorescent protein (EGFP) fusion of annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells. Detection can be analyzed by flow cytometry or by fluorescence microscopy with a FITC filter. EGFP is brighter and more photo-stable than other fluorescent reagents. The kit can differentiate apoptosis vs necrosis when performing both annexin V-EGFP and PI staining.

‧ Detection method- Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy
‧ Sample type- Living cells (suspension and adherant)
‧ Species reactivity- Mammalian
‧ Applications-Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits
‧ Simple one step staining procedure in 10 minutes
‧ Fast and convenient
‧ EGFP is a bright and photo-stable green fluorescent protein and the kit can differentiate apoptosis vs necrosis when performing both annexin V-EGFP and PI staining

Kit Contents:
Annexin V-EGFP
1X Binding Buffer
Propidium Iodide (PI)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Storage

Store at +4ºC. Product has an expected shelf life of 12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Annexin V-EGFP Apoptosis Detection Kit. GTX85588

GTX85588 ICC/IF Image

Detection of Apoptosis with Annexin V-EGFP. Apoptosis was induced in Jurkat cells by camptothecin (2 mM) for various times as indicated. Cells were collected and incubated with Annexin V-EGFP for 5 minutes according to the kit instructions. Results were analyzed by fluorescence microscopy (top panel) and flow cytometry (bottom panel).

Annexin V-EGFP Apoptosis Detection Kit. GTX85588

GTX85588 Image

10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.

Annexin V-EGFP Apoptosis Detection Kit. GTX85588

GTX85588 ICC/IF Image

Detection of Apoptosis with Annexin V-EGFP. Apoptosis was induced in Jurkat cells by camptothecin (2 mM) for various times as indicated. Cells were collected and incubated with Annexin V-EGFP for 5 minutes according to the kit instructions. Results were analyzed by fluorescence microscopy (top panel) and flow cytometry (bottom panel).

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Package List Price ($)
$ 439