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Annexin V-FITC Apoptosis Detection Kit

Annexin V-FITC Apoptosis Detection Kit. GTX85591
Annexin V-FITC Apoptosis Detection Kit. GTX85591

Cat. No. GTX85591

Reference 6

Application

 ICC/IF, FACS

Reactivity

 Human
Package
100 assay ($529)
See all Annexin V products
PRODUCT

Summary

Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy. The kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining.

‧ Detection method- Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy
‧ Sample type- Living cells (suspension and adherant)
‧ Species reactivity- Mammalian
‧ Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits
‧ Simple one step staining procedure in 10 minutes
‧ Fast and convenient
‧ Kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining

Kit Contents:
Annexin V-FITC
1X Binding Buffer
Propidium Iodide (PI)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

PROPERTIES

Storage

Store at +4ºC. Product has an expected shelf life of 12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Annexin V-FITC Apoptosis Detection Kit. GTX85591

GTX85591 Image

Annexin V-FITC Apoptosis Detection Kit. GTX85591

GTX85591 ICC/IF Image

Detection of Apoptosis with Annexin V-EGFP. Apoptosis was induced in Jurkat cells by camptothecin (2 mM) for various times as indicated. Cells were collected and incubated with Annexin V-EGFP for 5 minutes according to the kit instructions. Results were analyzed by fluorescence microscopy (top panel) and flow cytometry (bottom panel).

REFERENCE

Application Reference

George TA et al. J Control Release 2021; Liposome-encapsulated anthraquinone improves efficacy and safety in triple negative breast cancer.
Ha CT et al. Carcinogenesis 2021; 42 (7) : 951-960 ID4 predicts poor prognosis and promotes BDNF-mediated oncogenesis of colorectal cancer.
Reactivity : Human
Livan Delgado-Roche et al. Front Pharmacol 2020; 11 : 592985 Polyphenolic Fraction Obtained From Thalassia testudinum Marine Plant and Thalassiolin B Exert Cytotoxic Effects in Colorectal Cancer Cells and Arrest Tumor Progression in a Xenograft Mouse Model.
Application : FACS
Reactivity : Human
Couder-Garc?a BDC et al. Front Oncol 2019; 9 : 1341 The Phytosterol Peniocerol Inhibits Cell Proliferation and Tumor Growth in a Colon Cancer Xenograft Model.
Application : FACS
Reactivity : Human
Han W et al. Oncol Lett 2018; 16 (2) : 2416-2426 Expression of miR-30c and BCL-9 in gastric carcinoma tissues and their function in the development of gastric cancer.
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Package List Price ($)
$ 529