Annexin V-FITC Apoptosis Detection Kit

Cat No. GTX85591

Application	ICC/IF, FACS
Reactivity	Human
Package	100 assay ($499)

See all Annexin V products

Datasheet
Reference

PRODUCT

Summary

Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy. The kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining.

‧ Detection method- Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy
‧ Sample type- Living cells (suspension and adherant)
‧ Species reactivity- Mammalian
‧ Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits
‧ Simple one step staining procedure in 10 minutes
‧ Fast and convenient
‧ Kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining

Kit Contents:
Annexin V-FITC
1X Binding Buffer
Propidium Iodide (PI)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
ICC/IF	Assay dependent
FACS	Assay dependent

Not tested in other applications.

PROPERTIES

Storage

Store at +4ºC. Product has an expected shelf life of 12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

GTX85591 Image

GTX85591 ICC/IF Image

Detection of Apoptosis with Annexin V-EGFP. Apoptosis was induced in Jurkat cells by camptothecin (2 mM) for various times as indicated. Cells were collected and incubated with Annexin V-EGFP for 5 minutes according to the kit instructions. Results were analyzed by fluorescence microscopy (top panel) and flow cytometry (bottom panel).