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Annexin V antibody [N2C3]

KO/KD
Anti-Annexin V antibody [N2C3] used in Western Blot (WB). GTX113384
Anti-Annexin V antibody [N2C3] used in Western Blot (WB). GTX113384
Anti-Annexin V antibody [N2C3] used in Western Blot (WB). GTX113384
Anti-Annexin V antibody [N2C3] used in Flow cytometry (FACS). GTX113384

Cat. No. GTX113384

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Annexin V products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

Mouse brain , HeLa , HepG2 , 293T

Predict Reactivity

Rat, Bovine, Dog, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Annexin V. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036238

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

annexin A5 , ANX5 , ENX2 , HEL-S-7 , PP4 , RPRGL3

Background

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Annexin V antibody [N2C3] used in Western Blot (WB). GTX113384

GTX113384 WB Image

KO/KD

Wild-type (WT) and Annexin V knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin V antibody [N2C3] (GTX113384) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin V antibody [N2C3] used in Western Blot (WB). GTX113384

GTX113384 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin V antibody [N2C3] (GTX113384) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin V antibody [N2C3] used in Western Blot (WB). GTX113384

GTX113384 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX113384 diluted at 1:1000

Anti-Annexin V antibody [N2C3] used in Flow cytometry (FACS). GTX113384

GTX113384 FACS Image

Annexin V antibody [N2C3] (GTX113384) detects Annexin V protein by flow cytometry analysis.
Sample: HeLa cell.
Black: Unlabelled sample was used as a control.
Red: Annexin V antibody [N2C3] (GTX113384) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

REFERENCE

Application Reference

Zhao C et al. Sci Signal 2016; 9 (428) : ra49 Mice lacking the intracellular cation channel TRIC-B have compromised collagen production and impaired bone mineralization.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169